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Targeted over-expression p27 by Lenti-SM22alpha-p27-EGFP Recombinant Lentiviral Vectors on Vascular Smooth Muscle Cells

JING Liang;XIE Min-jie;YU Zhi-yuan;LV Jia-gao;WANG Wei;Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology;  
Objective:To construct Lenti-SM22alpha-p27-EGFP recombinant lentiviral vectors targeting vascular smooth muscle cells(VSMCs) and to investigate the impact on VSMCs proliferation by cell cycle analysis. Methods: Recombinant lentiviruses were constructed. The viruses were transfected into VSMCs and vascular endothelial cells(VECs). Results: SM22α-p27 lentiviruses exhibited effective and specific inhibition on VSMCs. SM22α promoter targeted p27 was able to be selectively over-expressed in VSMCs and to inhibit proliferation by cell cycle arrest at G0/G1. Conclusion: Recombinant lentiviruses driven by SM22α promoter could selectively infect VSMCs, thus lead to p27 protein over-expression and G0/G1 arrest.
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