Laser Speckle Contrast Imaging of Collateral Blood Flow during Cerebral Infarction in Rats
MIN Zhe;LI Chang-sheng;FANG Yu;QIU Zhan-dong;ZHANG Su-ming;Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology;
Objective:To study the collateral blood flow and focal cerebral blood flow(CBF) in middle cerebral artery occlusion(MCAO) rats by laser speckle imaging(LSI). Methods: A permanent MCAO(pMCAO) model was generated in male SD rats(n=9) by the intraluminal filament technique. LSI was used to measure CBF and collateral blood flow in the ischemic cortical area and MCA territory after 90 min. The brain was stained with TTC to determine the infarct size 24 h later. Results: CBF was decreased in both the hemispheric cortical area and MCA territory in the first 15 min and increased blood flow velocity was observed in some animals later. Conclusion: LSI has high spatial and temporal resolution, and offers visual imaging.