Effect of Transplantation of Bone Mesenchymal Stem Cells transfected with BDNF on Alzheimer Disease Rats
ZHAO Hui-xin;ZHAO Gang-yong;ZHANG Ping;Department of Neurology,Affiliated Hospital of Hebei University;
Objective:To study the changes of N-methyl-D-aspartate receptor 1(NMDAR1) and learning and memory function after transplanting bone mesenchymal stem cells(MSCs) modified with brain-derived neurotrophic factor(BDNF) gene in brain of Alzheimer disease(AD) rats models. Methods: Fifty healthy SD rats were randomly divided into 5 groups with equal number in each group, groups normal, sham, AD, MSCs, and BDNF. The normal group had received no treatment; and the AD models were established in the other 4 groups. The MSDs and the BDNF groups were injected by 10 μL(about 5×106) MSCs or MSCs modified with BDNF gene respectively. The learning and memory abilities of the rats were evaluated with Morris water maze test. The expression of NMDAR1 was detected by immunohistochemistry. Results: The average escape latency(AEL) was significantly shorter and the frequency of crossing the platform was significantly increased in the MSCs and BDNF groups compared with those in the AD group. The rats in the BDNF group showed greater learning and memory function improvement and higher expression of NMDAR1 compared with those in the MSCs group(P 0.01). Conclusion: Bone MSCs modified with BDNF gene could improve the memory and learning of AD rats and up-regulate the expression of NMDAR1 in the hippocampus.