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《Neural Injury and Functional Reconstruction》 2014-06
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Effects of Nogo-A Antisence Oligodeoxynucleotides on Signal Pathway of PKC-Rho A in Rats with Cerebral Ischemia-Reperfusion

CHANG Li-ying;SHEN Zheng-lin;HE Xiao-ming;LIANG Yan-ling;ZHOU Hong-bin;MAO Chun;ZHANG Su-ming;Department of Neurology, Xiangyang Central Hospital, The First Affiliated Hospital, Hubei University of Arts and Science;  
Objective: To investigate the effects of Nogo-A antisence oligodeoxynucleotides on signal pathway of PKC-Rho A after cerebral ischemia-reperfusion in rats. Methods: Forty rats were randomly assigned to groups of sham-operated(A), reperfusion 6 h(B), reperfusion 24 h(C), reperfusion 72 h(D), reperfusion 1 week(E),reperfusion 2 weeks(F), Nogo-A 72 h(G) and Nogo-A 1 week(H), with 5 rats in each group. The middle cerebral artery occlusion(MCAO) models was established. The group A was not induced ischemia, and the groups B, C, D,E, F were reperfused with 6 h, 24 h, 72 h, 1 week, 2 weeks respectively after ischemia. The groups G, H were given intracerebroventricularly with Nogo-A antisence oligodeoxynucleotides after the ischemia, then reperfused with72 h or 1 week respectively. RT-PCR was used to determine the changes of PKCγ and Rho A m RNA expression while the Western blot technique was used to detect the PKCγ and Rho A protein expression in the periphery of infarction cerebral cortex. Results: The expression level of PKCγ m RNA in group B were obviously increased compared with group A(P0.01) and group C returned to normal(P0.05). The expression of PKCγ m RNA elevated again in group D(P0.01). It was highest in group E(P0.01). The expression of PKCγ m RNA in group H were significantly declined compared with group E(P0.05). The expressions of Rho A m RNA in group B, D,were higher than those in group A(P0.01). In group G and H, it was significantly declined compared with group D and E(P0.05). The expression of PKCγ protein were higher in group B,D than that in group A(P0.05). The increase was significant in group E(P0.01). The expression of PKCγ protein in group G and H were significantly declined in comparison with group D and E(P0.05). The expression of Rho A protein were increased in group B, D, E compared with that of group A(P0.05), which was significantly declined tin group G and H compared with group D and E(P0.05). Conclusion: The expression of PKCγ and Rho A gene showed two peaks and the second increase was coincident with the change of Nogo-A after cerebral ischemia-reperfusion. Intracerebroventricular injection of Nogo-A antisence oligodeoxynucleotides can inhibit this increase,suggesting that Nogo-A is involved in the activation of PKC/Rho A signal pathway.
【CateGory Index】: R743.3
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Chinese Journal Full-text Database 2 Hits
1 CHANG Li-ying~* LI Dan~* ZHANG Su-ming~* SHIQi-jia~* LI Luo-qing~* ZHANG Min~* YIN Xiao-ping~* *Department of Neurology,Xiangfan Hospital,Tongji Medical.College,Huazhong University of Science and Technology,Xiangfan 441021,China;Expressions of Nogo-A and its receptor at mRNA and protein levels after cerebral ischemiareperfusion in rats[J];Chinese Journal of Neuromedicine;2006-09
2 Chang Liying, Yin Xiaoping, Li Luoqing, et al. Department of Neurology,Tongji Hospital,Tongji Medical College, Huazhong Science and Technology University, Wuhan 430030;The effect of antisence oligodeoxynucleotides by intra-cerebroventricular injection on expression of Nogo-A mRNA and protein in rats[J];Stroke and Nervous Diseases;2006-01
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