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《Neural Injury and Functional Reconstruction》 2014-06
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Effects of Nogo-A Antisence Oligodeoxynucleotides on Signal Pathway of PKC-Rho A in Rats with Cerebral Ischemia-Reperfusion

CHANG Li-ying;SHEN Zheng-lin;HE Xiao-ming;LIANG Yan-ling;ZHOU Hong-bin;MAO Chun;ZHANG Su-ming;Department of Neurology, Xiangyang Central Hospital, The First Affiliated Hospital, Hubei University of Arts and Science;  
Objective: To investigate the effects of Nogo-A antisence oligodeoxynucleotides on signal pathway of PKC-Rho A after cerebral ischemia-reperfusion in rats. Methods: Forty rats were randomly assigned to groups of sham-operated(A), reperfusion 6 h(B), reperfusion 24 h(C), reperfusion 72 h(D), reperfusion 1 week(E),reperfusion 2 weeks(F), Nogo-A 72 h(G) and Nogo-A 1 week(H), with 5 rats in each group. The middle cerebral artery occlusion(MCAO) models was established. The group A was not induced ischemia, and the groups B, C, D,E, F were reperfused with 6 h, 24 h, 72 h, 1 week, 2 weeks respectively after ischemia. The groups G, H were given intracerebroventricularly with Nogo-A antisence oligodeoxynucleotides after the ischemia, then reperfused with72 h or 1 week respectively. RT-PCR was used to determine the changes of PKCγ and Rho A m RNA expression while the Western blot technique was used to detect the PKCγ and Rho A protein expression in the periphery of infarction cerebral cortex. Results: The expression level of PKCγ m RNA in group B were obviously increased compared with group A(P0.01) and group C returned to normal(P0.05). The expression of PKCγ m RNA elevated again in group D(P0.01). It was highest in group E(P0.01). The expression of PKCγ m RNA in group H were significantly declined compared with group E(P0.05). The expressions of Rho A m RNA in group B, D,were higher than those in group A(P0.01). In group G and H, it was significantly declined compared with group D and E(P0.05). The expression of PKCγ protein were higher in group B,D than that in group A(P0.05). The increase was significant in group E(P0.01). The expression of PKCγ protein in group G and H were significantly declined in comparison with group D and E(P0.05). The expression of Rho A protein were increased in group B, D, E compared with that of group A(P0.05), which was significantly declined tin group G and H compared with group D and E(P0.05). Conclusion: The expression of PKCγ and Rho A gene showed two peaks and the second increase was coincident with the change of Nogo-A after cerebral ischemia-reperfusion. Intracerebroventricular injection of Nogo-A antisence oligodeoxynucleotides can inhibit this increase,suggesting that Nogo-A is involved in the activation of PKC/Rho A signal pathway.
【CateGory Index】: R743.3
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 CHANG Li-ying~* LI Dan~* ZHANG Su-ming~* SHIQi-jia~* LI Luo-qing~* ZHANG Min~* YIN Xiao-ping~* *Department of Neurology,Xiangfan Hospital,Tongji Medical.College,Huazhong University of Science and Technology,Xiangfan 441021,China;Expressions of Nogo-A and its receptor at mRNA and protein levels after cerebral ischemiareperfusion in rats[J];Chinese Journal of Neuromedicine;2006-09
2 Chang Liying, Yin Xiaoping, Li Luoqing, et al. Department of Neurology,Tongji Hospital,Tongji Medical College, Huazhong Science and Technology University, Wuhan 430030;The effect of antisence oligodeoxynucleotides by intra-cerebroventricular injection on expression of Nogo-A mRNA and protein in rats[J];Stroke and Nervous Diseases;2006-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Jian-zhong1,JING Li1,2,GUO Feng-ying1,MA Yi1,WANG Yi-li2(1Department of Pathology,Ningxia Medical College,Yinchuan 750004,2Institute of Immunopathology,School of Life Science & Technology,Xi'an Jiaotong University,Xi'an 710061,China);Phosphorylation of extracellular signal-regulated protein kinase induced neuronal death under brain ischemia in diabetic rats[J];Acta Academiae Medicinae Militaris Tertiae;2007-24
2 WANG Rui-xue;LIU Lian;WANG Zhi-yong;State Key Lab.of New Drug & Pharmaceutical Process,Shanghai Institute of Pharmaceutical Industry,China State Institute of Pharmaceutical Industry;;Progress of neuroprotective drugs and their potential molecular targets in the treatment of ischemic stroke[J];World Clinical Drugs;2013-12
3 Cheng Chuang;Qin Xinyue;Guo Jia;Yan Weiping;Xu Guanghui;Bai Yongsheng;Department of Neurology,Chongqing Key Laboratory of Neurology,First Affiliated Hospital,Chongqing Medical University;;Fibulin-5 over-expression protects blood brain barrier in rats after cerebral ischemia/reperfusion injury[J];Journal of Third Military Medical University;2014-13
4 WANG Jing1,2,WANG Chengyao1,ZHANG Junjian31Dept.of Anesthesiology,3Dept.of Neurology,Zhongnan Hospital of Wuhan University,Wuhan 430071,China 2Dept.of Anesthesiology,Jiangyin People's Hospital of Jiangsu Province,Jiangyin 214400,China;Effect of Heme Oxygenase-1 Gene on Cognitive Function and Oxidative Stress During Chronic Cerebral Ischemia in Rats[J];Medical Journal of Wuhan University;2008-01
5 WANG Fei,et al.(Ningxia Medical University,Yinchuan 750004,China);The effect of c-Jun NH_2-terminal kinase on hyperglycemia-exaggerated cerebral ischemia damage[J];Ningxia Medical Journal;2010-12
6 Zou Haojun;Lin Lili;Sun Xuezhu;Wei Wei;Department of Pharmacology,Wuxi Higher Health Vocational Technology School;West Anhui Health Vocational College;Anhui HengXing Drug Research Institute;;Protection effects of methanesulfonic acid cinepazideon on brain ischemia reperfusion in rats[J];Journal of Nanjing Medical University(Natural Sciences);2013-10
7 YUAN Wei;WANG Yao-hui;MENG Lan-qing;Department of Neurology,Affiliated Hospital of Guilin Medical college;;Nogo-A gene polymorphism and genetic susceptibility in patients with ischemic stroke[J];Chinese Journal of Geriatric Heart Brain and Vessel Diseases;2014-07
8 HU Qian,WANG Li,JIANG Wei(Department of Anesthesiology,The Sixth People's Hospital,Shanghai Jiaotong University,Shanghai 200233,China);Effect of Ketamine on c-fos Gene Expression in Neuronal PC12 Cells Line Induced by Glutamate[J];Journal of Shanghai Jiaotong University(Medical Science);2006-02
9 YAN Liu-shi*,ZHU Yao-min,WANG Wei,YUAN Wei,JING Gui-xia(Dept of Anesthesiology,First Affiliated Hospital of Medical College of Xi'an Jiaotong University,Xi'an 710061,China);Effects of subanesthetic dose of ketamine preconditioning on postoperative cognitive function in the elderly patients[J];Journal of Shanxi Medical University;2012-02
10 AI Rong,WU Biao,SUN Ming-ming (Department of Pediatrics,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou Province,China);Expressions of Nogo-A mRNA and Nogo Receptor mRNA in Hippocampus of Immature Rats after Febrile Seizures[J];Journal of Applied Clinical Pediatrics;2008-18
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