Effect of VEGF Monoclonal Antibody on Proliferation,Invasion and Migration of C6 Glioma Cells in vitro
LI Jun;YE Liang;WANG Fan;Department of Neurosurgery,Mianyang 404 Hospital;
Objective: To observe the effect of vascular endothelial growth factor(VEGF) monoclonal antibody on C6 glioma cells' proliferation, migration and invasion in vitro. Methods: Co Cl2 was added in culture medium to imitate hypoxic culture condition. C6 glioma cells were treated by VEGF antibody with different concentrations(0.1 μg/m L and 1 μg/m L) under normoxic or hypoxic conditions. Cell proliferation was detected by MTT assay. The ability of cell migration and invasion were evaluated by Transwell testing assay. The expression of HIF-1α protein, FAK/Pyk2 total protein and level of FAK-Tyr397/Pyk2-Tyr402 phosphorylation were detected by Western blot. Results: Treatment with 200 μmol/L Co Cl2 significantly inhibited the proliferation of C6 glioma cells(P0.05). 10 μg/m L VEGF antibody significantly inhibited the proliferation of C6 glioma cells(P0.05). Compared to the normoxic condition, hypoxiia increased the migration and invasion of C6 cells(P0.05).Under the normoxic and hypoxic condition, the VEGF antibody can increase the migration and invasion of C6(P0.05). Compared to the normoxic condition, hypoxia can enhance the migration and invasion by VEGF antibody to C6 cell(P0.05). The expression of total FAK protein was decreased in C6 cells by VEGF antibody only in the 1μg/ml concentration(P0.05). Under hypoxic condition, the phosphorylation levels of Pyk2-Tyr402 was increased in C6 cells after VEGF antibody treatment(P0.05). Conclusion: Under hypoxic condition, VEGF antibody can increase the phosphorylation levels of Pyk2-Tyr402, which contribute to the invasion and migration of C6 cells.