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《Foreign Medical Sciences Section On Neurology & Neurosurgery》 2005-04
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Effect of dendritic cells pulsed with Tα146-162 on the expression of Crry, CD59a, and CD59b mRNA in experimental autoimmune myastheia gravis

LI Shui-Xian, YANG Huan, XIAO Bo, HU Jue, HUANG Jie. Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China  
Objective To study the expression of Crry, CD59a and CD59b mRNA in the muscle of mice immunized with T-AChR and of the immunized mice injected with immature myeloid dendritic cells (iMDCs) pulsed with immunodominant peptide T-AChRα146-162 (Tα146-162), and to investigate the effect of iMDC pulsed with Tα146-162 (Tα146-162- iMDC) induced antigen specific tolerance and the effect of the tolerance on the expression of Crry, CD59a and CD59b mRNA. Methods Precursor cells from mice bone marrow were cultured and differentiated in medium supplemented with GM-CSF and IL-10 for six days. Immature myeloid DCs were collected and pulsed with Tα146-162. 30 C57BL/6 mice were randomly divided into model group (A, n=12), intervention group (B, n=12) and normal control group (C, n=6). Mice of group A and B were immunized with T-AChR in CFA. Mice of group B were subcutaneously injected with Tα146-162-iMDC. Mice of group A and C received injections of PBS. Mice were evaluated for Christadoss standard, lymphocyte proliferation test was used to confirm the onset of experimental autoimmune myastheia gravis (EAMG). On the day 90 after the first immunization, mice were sacrificed, lymphocyte proliferation was assayed and Crry, CD59a and CD59b mRNA were detected by RT-PCR method. Results The average clinical scores of group A and group B mice were 1.67±1.15 vs 0.33±0.65 (P0.01), and the accumulated incidences were 75% vs 25% (P0.05). Compared with group A mice, group B mice had a significantly reduced lymphocyte proliferative response (P0.01) . The expression of Crry mRNA and CD59a mRNA decreased significantly in muscle of group A mice compared with that of group C mice (P0.05). The decrease was inhibited in mice of group B injected with Tα146-162-iMDC, but the expression of Crry mRNA was still lower than that of group C mice (P0.05). There was no significant difference in CD59a mRNA expression in muscle between group B and C (P0.05). CD59b mRNA could not be detected in the muscle of three groups. Conclusions Tα146-162-iMDC could prevent EAMG. The decrease of Crry mRNA and CD59a mRNA may involve in the pathogenesis of EAMG but CD59b could not correspond with it. The protective effect of Tα146-162-iMDC could be associated with the increased expression of Crry mRNA, CD59a mRNA in muscle and the antigenic specific suppression of lymphocyte proliferation.
【CateGory Index】: R392
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Chinese Journal Full-text Database 10 Hits
1 GUO Xiao, PAN Ling (Department of Hematology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China);Effect of ERK pathway inhibitor U0126 on cell cycle of leukemia K562 cell lines[J];Acta Academiae Medicinae Militaris Tertiae;2008-05
2 Fangyun;GuoYiqing,et al.(Children’s Hospital,Shanghai Medical University,Shanghai 200032);Hepatitis B virus associated nephritis in adults and children[J];CHINESE JOURNAL OF INFECTIOUS DISEASES;1996-02
3 CHEN Yong-bing YAN Mao-lin GONG Jian-ping XIA Ren-pin LIU Li-xin LI Ning LU Shi-chun ZHANG Jing-guang ZENG Dao-bing XIE Jian-guo YANG Jia-yin YAN Lü-nan Department of General Surgery,Second Hospital,Chongqing University of Medical Sciences,Chongqing 400010,ChinaDepartment of General Surgery,West China Hospital,Sichuan University,Chengdu 610041,ChinaLiver Transplantation Centre,Beijing You-an Hospital,Capital University of Medical Sciences,Beijing 100069,ChinaLiver Transplantation Centre,General Hospital of People's Liberation Army,Beijing 100852,China;Establishment of hepatocellular carcinoma multidrug resistant monoclone cell line HepG2/mdr1[J];中华医学杂志(英文版);2007-08
4 ZhangAip-ing,ZhangXun,HouFanfan,etal.Departmentofnephrology,GeneralHospitalofJinanCommand,Jinan250031;DetectionofHBVDNAinkidneybyusinginsituhybridizationandinsitupolymerasechainreaction[J];CHINESE JOURNAL OF NEPHROLOGY;1996-05
5 WU Sheng-hua, LU Chao, DONG Ling, ZHOU Guo-ping, JIANG Xin-you. Department of Pediatrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;Lipoxin A_4 inhibits CTGF-induced production of RANTES in mesangial cells[J];Chinese Journal of Microbiology and Immunology;2005-11
6 CHEN Nan, WANG Zhaohui, REN Hong, et al. Department of Nephrology, Ruijin Hospital, Shanghai 2 nd Medical University,Shanghai 200025, China;The presence and significance of hepatitis B virus DNA and RNA in kidney[J];National Medical Journal of China;2001-21
7 LIU Hong-bao,ZHAO Feng,YU Yan,et al.Department of Nephrology,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,China;Effects of bone marrow mesenchymal stem cells on the expression of Bcl-2 and Fas/FasL proteins in renal tubular cells during ischemia-reperfusion injury in rat[J];Chinese Journal of Critical Care Medicine;2008-01
8 Jian Guan Xiao-Ping Chen Hong Zhu Shun-Feng Luo Bin Cao Lei Ding Hepatic Surgery Center,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei Province,China;Involvement of extracellular signal-regulated kinase/mitogenactivated protein kinase pathway in multidrug resistance induced by HBx in hepatoma cell line[J];世界胃肠病学杂志(英文版);2004-23
9 Nian-Song Wang, Department of Nephrology, The Sixth Affiliated Hospital of Shanghai Jiaotong University, 600 Yushan Road, Shanghai 200233, China Zhao-Long Wu, Lu-Tan Liao, Department of Nephrology, Zhong Shan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China Yue-E Zhang, Department of Pathology, Fudan University, 136 Yixue Road, Shanghai 200032, China;Existence and significance of hepatitis B virus DNA in kidneys of IgA nephropathy[J];世界胃肠病学杂志(英文版);2005-05
10 Na Lin, Hong-Ying Chen, Dan Li, Sheng-Jun Zhang, Zhi-Xin Cheng, Xiao-Zhong Wang, Department of Gastroenterology, Affiliated Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China;Apoptosis and its pathway in X gene-transfected HepG_2 cells[J];世界胃肠病学杂志(英文版);2005-28
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