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《Foreign Medical Sciences Section On Neurology & Neurosurgery》 2005-04
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Establishment of an improved rat model of Parkinson's disease

ZHOU Hou-guang, BAO Yuan-cheng, LU Jian-ming. Department of Neurology,Affiliated Changshu Hospital, edical College of Yangzhou University, Changshu, Jiangshu 215500, China  
Objective To study the method of increasing success rate of Parkinson's disease (PD) rat model establishment. Methods Ninety SD rats were administered with stereotaxic microinjection of 6-OHDA into the left substantia nigra compact part (SNC) and the ventral tegmental area (VTA) of the midbrain. The changes of behaviors, nigral antioxidation index, mitochondrial respiration-chain function, and the morphology of nigra dopaminergic neuron (NDN) were observed after 6-OHDA injection. Results PD rat model was successfully established in 64 rats, with success rate of 71%. In the rat models, the contents of ROS and MDA obviously increased but GSH-Px activity significantly decreased. Dysfunction of mitochondrial respiration-chain was noted in substantia nigra. The immunohistochemical staining showed a significant reduction of TH-positive neurons in the 6-OHDA injection side compared with the opposite side. Electron-microscope demonstrated that apoptosis, degeneration and necrosis existed extensively in NDN. Conclusions PD rat model with high success rate was established by two site injection of 6-OHDA which could damage NDN probably through increasing oxidative stress, interfering mitochondrial respiration-chain function, and inducing apoptosis and necrosis.
【Fund】: 安徽省自然科学基金(98JL086)
【CateGory Index】: R-332
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【References】
Chinese Journal Full-text Database 3 Hits
1 JIA Xin1, YANG Zhi-Fu2, ZHOU Jun3, ZHANG Jin-Ping1, ZHANG Juan1, JIANG Wei-Feng1, CHEN Jian-Zong1 1Institute of Traditional Chinese Medicine, 2Department of Pharmacy, Xijing Hospital, 3 Department of Chemistry, School of Pharmacy, Fourth Military Medical University, Xi'an 710033, China;Pharmacodynamics of Pingchan Potion on Parkinson's Disease[J];Journal of the Fourth Military Medical University;2008-08
2 Liu Yunlong1, Yang Ning1, Zeng Shuilin1, Liu Fangzhou2, Hua Julan1, Li Fengfei1, Dong Bo1(1Department of Neurobiology, Southeast University, Nanjing 210009; 2The Tumour Hospital of Jiangsu, Nanjing 210009);THE EXPRESSION OF GFR-α1 IN THE SUBSTANTIA NIGRA OF RATS WITH PARKINSON DISEASE[J];Chinese Journal of Neuroanatomy;2007-01
3 JIA Xin~1 CHEN Jian-zong~1 YANG Zhi-fu~2 CHEN Hong~1 ZHANG Jin-ping~1 ZHANG Juan~1 JIANG Wei-feng (1 Institute of Traditional Chinese Medicine,2 Department of Pharmacy,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,China);Neuroprotective effect of tetrahydroxystilbene glucoside on the rat model of Parkinson's disease[J];Chinese Journal of New Drugs;2008-09
【Citations】
Chinese Journal Full-text Database 3 Hits
1 Zhou Mingfu;Zhang Hua;Wu Jinliang;Guo Zhangsheng;LinLing;Tang Shanjun;Zhou Xiangting(Department of Anatomy,Henan Medical University,Zhengzhou 450052);Establishment of animal model for Parkinson's disease[J];JOURNA OF HENAN MEDICAL UNIVERSITY;1994-04
2 Zhou Guoqin Zhou Xiaoda Li Ningli Shen Baiha Deprtment of Neurology, Renji Hospital, Shanghai Second Medical University.Shanghai 200001;A study on apoptosis in PC12 cell induced by 6-Hydroxydopamine[J];Journal of Brain and Nervous Diseases;1997-05
3 ZHOU Hou-guang ,LU Jian-ming,BAO Yuan-cheng,et al. The Department of Neurology,The 2nd Hospital of Changsu,Jiang shu 215500;The establishment and evaluation of 6-OHDA-lesioned rat model for parkinson's disease[J];Chinese Journal of Behavioral Medical Science;2002-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SU Ping, LI Yan-hua, ZHENG Min, et al. Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;Establishment of an improved rat model of Parkinson's disease[J];Guangdong Medical Journal;2006-07
2 YIN Feng,TIAN Zeng-min,ZHAO Quan-jun,WANG Ya-ming,YU Xue,LIU Shuang(Department of Neurosurgery,Naval General Hospital,Beijing 100037,China);Experimental Study on Establishment of Rats Model of Parkinson Disease by 6-OHDA Injection[J];Journal of Naval General Hospital;2007-02
3 LU Jian-ming, ZHOU Hou-guang,BAO Yuan-cheng, et al. Department of Neurology, Affiliated Changshu Hospital of Medical College, Yangzhou University, Changshu 215500,China;Experimental study on cytotoxicity of levodopa in Parkinson's disease rat[J];Journal of Clinical Neurology;2004-05
4 Lv Shengqing,Zhang Kecheng,Cai Wenqin,He Jiaquan,Liu Shiyong Department of Neurosurgery,Xinqiao Hospital,Third Military Medical College,Chongqing 400037 * Department of Histology and Embryology,Third Military Medical College,Chongqing 400038;Experimental study of Nao Gong Fu De in treatmint of Parkinson's disease[J];CHINESE JOURNAL OF STEREOTACTIC AND FUNCTIONAL NEUROSURGERY;1999-02
5 HUO Juan,WANG Wei,SUN Lijun,et alDept of Neurology,Affiliated Hospital of Inner Mongolia Medical College,Huhhot(024000),China;Study of Cortical threshold of Motor evoked potentials by transcranial magnetic stimulation for model rats with Parkinson disease[J];Journal of Clinical Electroneurophysiology;2007-06
6 Zhang Wei,Yang Hui.Department of Neurosurgery,Xinqiao Hospital,Third Military Medical University of PLA,Chongqing 400037,China;Establishment and Evaluation of Parkinson's disease model of rat with 6-OHDA[J];Sichuan Medical Journal;2007-08
7 Zhou Hou guang, Lu Jian ming,Bao Yuan cheng,Yang Zhi yong,You Nian xing Zhou Hou guang,Lu Jian ming,You Nian xing, Department of Neurology,Changshu Hospital, Medical College of Yangzhou University, Changshu 215500, Jiangsu Prov ince, China Bao Yuan cheng,Department of Neurology, First Affiliated Hospital, Anhui Coll ege of Traditional Chinese Medicine,Hefei 230031,Anhui Province, China Yang Zhi yong,Sun Yat sen University of Medical Sciences, Guangzhou 510001,G uangdong Province, China;Protective effect of reduced glutathione in treating rat model of Parkinson di sease combined with dementia[J];Chinese Journal of Clinical Rehabilitation;2005-01
8 ZHOU Hou-guang,BAO Yuan-cheng,LU Jian-ming,et al. (Department of Neurology,The Affiliated Changshu Hospital of Yangzhou University Medical College,Changshu 215500,China);The experimental study of glutathione on cyto-protection function in Parkinson disease rat[J];Journal of Apoplexy and Nervous Diseases;2005-03
9 Lu Shengqing, Zhang Kecheng, Liu Haipeng, et al Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037;Change of the rotational behavior after implantation of microencapsulated PC12 cells in PD rats[J];CHINESE JOURNAL OF BEHAVIORAL MEDICAL SCIENCE;2000-02
10 ZHOU Hou-guang ,LU Jian-ming,BAO Yuan-cheng,et al. The Department of Neurology,The 2nd Hospital of Changsu,Jiang shu 215500;The establishment and evaluation of 6-OHDA-lesioned rat model for parkinson's disease[J];Chinese Journal of Behavioral Medical Science;2002-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Wang Yuanjing,Shen Guoming,Wang qinmao (Department of Combination of Chinese and Western Medicin, Anhui College of TCM,Hefei 230038,China);THE PROTECTIVE EFFECTS OF LIGUSTRAZINE ON ACUTE CEREBRAL ISCHEMIA REPERFUSION INJURY IN RATS[J];JOURNAL OF ANHUI TRADITIONAL CHINESE MEDICAL COLLEGE;2000-01
2 CHENJian-zong,LIGang,LIANG Hong-juan(Institute ofTraditional Chinese Medicine,Xijing Hospital,theFourth Military Medical University,Shaanxi Xi′an 710032,China);Effect of Peibu Ganshen Recipe on Changes of Monoamine Transmitters of Brain in Mice of Parkinson′s Disease Model[J];Journal of Anhui Traditional Chinese Medical College;2005-05
3 PU Xiao Ping 1, LI Xiao Rong 1, LI Hui Nong 1, TU Peng Fei 2, SONG Zhi Hong 2, LI Chang Ling 1 (1. Department of Molecular and Cellular Pharmacology, 2. Department of Natural Medicines, Peking University School of Pharmaceutical Sciences, B;Campneoside Ⅱ of Cistanche tubulosa (Schenk) R. Wight protects neurons from apoptosis induced by neurotoxin 1 methyl 4 phenylpyridinium (MPP +)[J];Journal of Beijing Medical University;2001-03
4 WANG Xue Mei △, FU Hong, LIU Geng Xin (Institute of Integrated of Traditional Chinese and Western Medicine, Peking University, Beijing 100034,China);Effect of Herba Epimedii and Fructus Lycii on mitochondrial DNA deletion, activity of respiratory chain enzyme complexes and ATP synthesis in aged rats[J];Journal of Beijing Medical University;2002-01
5 WU Qiang 1, LI Yun-peng 1, YANG He-ping 2, LE Wei-dong 3, DONG Zhao-jun 1 ( 1Department of Military Toxicology, College of Preventive Medicine, 2Department of Respiratory Diseases, Southwest Hospital, Third Military Medical University, Chongqing 400038, China; 3Department of Neurology, Baylor College of Medicine, TX77030, USA);Effects of long-term rotenone intoxication on the monoamine neurotransmitters, NO, and NOS in rat striatum[J];Acta Academiae Medicinae Militaris Tertiae;2004-22
6 TIAN Ji Yu 1, CHEN Jian Zong 1, CHEN Xiao Li 2, YANG Hao 3 1Department of Traditional Chinese Medicine, 2Department of Pharmacy, Xijing Hospital, 3PLA Institute of Neurobiology, Fourth Military Medical University, Xian 710033, China;Effects of Cistanche on apoptosis of PC12 cells[J];Journal of The Fourth Military Medical University;2004-10
7 XI Shu-Fang~1, CHEN Jian-Zong~1, WANG Bai-Ren~2, YANG Hao~2, GAO Jian-Yuan~3, TIAN Ji-Yu~1, KANG Xiao-Gang~1~1Research Center of Traditional Chinese Medicine, Laboratory of Integrated Traditional Chinese Medicine and Western Medicine for Encephalopathy in Elderly, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China, ~2PLA Institute of Neurobiology, ~3Department of Geriatrics, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China;Protective effect of EGB761 on embryonic rat mesencephalic dopaminergic neurons in vitro from MPP~+-induced injury[J];Journal of the Fourth Military Medical University;2006-01
8 Liu Wenguo, He Hongwen, Song Dage, Xie Yao. Department of Anatomy, Foshan Employee Medical College, Foshan 528000 China;Effect of the peoniflorin in culture neurons of the hippocampus and septal area of rat[J];Anatomy Research;2002-04
9 LI Yong-mei1 ,LI Xia2,3,ZHOU Zhao-li3(1.146 of Warehouse,State Food and Drug Administration,Shijiazhuang 050071,China;2.Department of Pharmacy,Ocean College of Shandong University at Weihai, Weihai 264209,Shandong Province,China;3.Department of Pharmacology,Shenyang Pharmaceutical University,Shenyang 110016,China);Proventive effects of Polygonum multiflorum Thunb.extract on nigrostriatal dopaminergic system lesion induced by paraquat in mice[J];The Chinese Journal of Clinical Pharmacology;2007-05
10 Zhu Xiaoqin, Zhang Duanlian, Hu Qisheng, et alDepartment of Histology and Embryology, School of Medici ne, Wuhan University, Wuhan 430071, China;Effects of Tetramethylpyrazine on C-fos Expression in Neuronal Cells in Rats with Epilepsis Induced by Penicillin[J];Journal of Hubei Medical University;2002-04
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 Jiang Yu1,2,Zeng Shuilin1,Lu Youyu2,Zhu Jianbao1,Li Tao1,Li Fengfei1,Xu Chunhua2(1.Institute of Neurobioloy,Medical College of Southeast University,Nanjing 210009;2.Department of Neurology,Yangzhou Hongquan Hospital Affiliated To Medical College of Yangzhou University,Yangzhou 225200,China);GDNF promotes to express Pitx3,Nurr1 and TH in transplanted midbrain-derived neural stem cells of Parkinson's disease model of rats[J];Chinese Journal of Neuroanatomy;2011-01
2 TAO Li-zhen1,LI Yan2,LI Xiao-feng1,LI Xiao-bing1,SUN Xin1,CHEN Jian-zong1(1 Research Center of Traditional Chinese Medicine,Xijing Hospital,Fourth Military Medical University,Xi'an 710032; 2 Pharmaceutical Preparation Section,Xijing Hospital,Fourth Military Medical University,Xi'an 710032);Effect of 2,3,5,4'-tetrahydroxystibene-2-o-β-D-glucoside in mitigation of MPP+-induced Apoptosis of PC12 Cells[J];Progress in Modern Biomedicine;2011-04
3 KAN Hong-wei1,WEI An-xiang2,BAO Guang-li3,LIANG Yan1,XU Peng-fu1,YANG Shi-you1(1 Anhui Institute of Materia Medica,Anhui Province Key Laboratory of Traditional Chinese Medicine Research and Development,Hefei 230022,China;2 School of Life Science,Anhui University,Hefei 230039,China;3 Dong Zhi Tang Pharmaceutical Anhui Co.,Ltd.,Huainan 232001,China);Effect of Huishengzaizao bolus in rat model of Parkinson's disease[J];Chinese Journal of New Drugs;2010-11
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Zhou Mingfu;Zhang Hua;Wu Jinliang;Guo Zhangsheng;LinLing;Tang Shanjun;Zhou Xiangting(Department of Anatomy,Henan Medical University,Zhengzhou 450052);Establishment of animal model for Parkinson's disease[J];JOURNA OF HENAN MEDICAL UNIVERSITY;1994-04
2 Zhou Guoqin Zhou Xiaoda Li Ningli Shen Baiha Deprtment of Neurology, Renji Hospital, Shanghai Second Medical University.Shanghai 200001;A study on apoptosis in PC12 cell induced by 6-Hydroxydopamine[J];Journal of Brain and Nervous Diseases;1997-05
3 Shao Yanping (Department of Anatomy);Intracerebral Grafting of Neuronal Cell Suspensions Correct the Damage of Substantia Nigra Ⅰ. Animal Model[J];;1986-02
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