Over-expression of bcl-xl gene has protective effects against occlusion of middle cerebral artery in transgenic mice
WANG Fu-Rong, JIANG Yong-Sheng, LIU Yan, XIAO Wen-Wu, ZHANG Su-Ming, ZHU Sui-Qiang. Tongji Hospital, Tongji Medical School, Huazhong Science and Technology University, Wuhan 430030, China
Objective To study the protective effects of the over-expression of bcl-xl gene on transgenic mice following permanent occlusion of middle cerebral artery and the possible mechanism.Methods Transgenic mice and wild type mice were subjeced to intraluminal occlusion of the middle cerebral artery. The neurological outcomes were evaluated 24 hrs later. The infarct volume at different times after occlusion were measured. The apoptosis of neuron was measured by TUNEL. The expression of bcl-xl before and after occlusion was detected with immunohistochemistry.Results The neurological function scores in transgenic mice were significantly lower than those in the wild type ones (P0.05). The number of neuron apoptosis in transgenic mice was significantly less than that in mild type mice (P0.05). The infarct volume of the transgenic mice was much less than that of the wild type mice at 3, 24 and 72 hrs after ischemia (P0.05). The bcl-xl expression increased after ischemia in transgenic mice and wild mice, but the bcl-xl expression was higher in transgenic mice before and after brain ischemia than that in the wild type mice (P0.05).Conclusions Over-expression of bcl-xl can significantly reduce the infarct volume and improve neurological function in transgenic mice, possibly through inhibiting the apoptosis of neuron.