Effect of fibrin on the expression of vascular endothelial growth factor in rat brain vascular endothelial cells
XUE Zheng, ZHANG Su-Ming. Department of Neurology,Tongji Hospital, Huazhong University of Science & Technology, Wuhan, Hubei 430030, China
Objective To investigate the effect of fibrin vascular endothelial growth factor (VEGF) protein and mRNA expressions in rat brain vascular endothelial cells. Methods VEGF concentration were measured by ELISA kit and VEGF mRNA was measured by real-time PCR in an in vitro model of in situ fibrin polymerization on rat brain vascular endothelial cells. Results Fibrin could induce VEGF expression. VEGF antigen concentration increased significantly in the 1.0 mg/ml media group compared to the low-dose fibrin groups and the control media group (P0.001). Fibrin-induced VEGF expression in rat brain vascular endothelial cells (VECs) increased as early as 2 hrs after fibrin stimulation, increased more significantly at 8 hrs, and peaked at 24 hrs (P0.005). Real-time PCR analysis showed that VEGF mRNA increased in the fibrin-treated rat brain VECs in a time-and dose-dependent fashion. Conclusions Fibrin can induce VEGF expression in rat brain VECs.