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《Foreign Medical Sciences》 2004-01
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Comparison of the results by three automatic immunoassay for determination of HBV markers in serum

CHEN Yu, LI Lan juan, LOU Bin, et al.The 1st Hospital of Medical School, Zhejiang University, Hangzhou,310003, China.  
Objective To discuss the agreement of three automatic immunoassay systems for determining HBV markers in serum. Methods A total of 120 serum samples were collected randomly from hospitalized patients determined for five HBV markers including HBsAg, Anti HBs, Anti HBc, HBeAg and Anti HBe by AxSYM, Elecsys 2010 and ARCHITECT i2000,respectively .The concordance rates by Elecsys 2010 and ARCHITECT i2000 were calculated and compared with that by AxSYM.Results The concordance rates (%) by Elecsys 2010 with AxSYM was 99.2% for HBsAg, 85.8% for Anti HBs, 87.5% for Anti HBc, 99.2% for HBeAg and 90.0% for Anti HBe, respectively,and the concordance rate by ARCHITECT i2000 with AxSYM was 100% for HBsAg, 96.7% for Anti HBs,91 7% for Anti HBc, 100% for HBeAg and 95.8% for Anti HBe, respectively.The correlation coefficient (r) of Elecsys 2010 with AxSYM was 0.90 for Anti HBs, lower than that of ARCHITECT i2000 with AxSYM( r =0.98).The concordance rate by Elecsys 2010 for the five HBV markers was 65.8% and that of ARCHITECT i2000 was 85.0%.Conclusion There were some discrepant results among three automated immunoassay systems for determining HBV markers in serum,mostly in HBV specific antibodies and low level antigens.The determining methods should be standardized furtherly.
【CateGory Index】: R446.6
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