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《Foreign Medical Sciences》 2004-01
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Clinical study of measurement parameters from Diana-5 fully automated blood cell analyzer

YANG Yan.Department of Laboratory Test, First aid Center of Chongqing City,Chongqing 400014, China.  
Objective To evaluate the clinical application value of measurement parameters from Diana 5 fully automated blood cell analyzer.Methods According to criterion of preliminary evaluation of quantitative clinical laboratory methods(EP10 T2) of National Committee for Clinical Laboratory Standards(NCCLS), the complete blood count (CBC) and 5 part difference were measured by Diana 5 fully automated blood cell analyzer. Then the results were statistically analyzed using Microsoft Excel 2000 and SAS software. Results The within run CV was less than 2% for WBC, RBC and HGB in samples of high and middle value.They were less than 3% in low value,and the between run CV was 3% within them.The within run and between run CV were 2% for MCV, and those of PLT were 4.5% and 5.2%.The toal CV was 2.5% for WBC, RBC, HGB and MCV, and that of PLT was 5%.The linearity correlation coefficients of WBC, RBC, HGB, HCT and PLT were 0.999 , 0.999, 0.999, 0.998 and 0.995,respectively.The bias of PLT was 3%,but the detection values of WBC, RBC, HGB and HCT were near to true value of quality control samples.The carryover of WBC was 1%, and those of RBC and PLT were 1%, and that of HGB and MCV was 0. Compared with by those microscope examination,correlation coefficients of Neu, Lym, Mon, Eos and Bas were 0.982, 0.956 , 0.842, 0.536 and 0.328, respectively.Conclusion The results of CBC by Diana 5 blood cell analyzer possess good linearity,and high preciseness and accuracy,and the carryover is lower.The results of 5 part difference are precise and accurate, suitable for screening abnormal blood cell.
【CateGory Index】: R446.1
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