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《International Journal of Laboratory Medicine》 2008-02
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Detection of the genomic mutations of the multidrug-resistant Mycobacterium tuberculosis by polymerase chain reaction-DNA sequencing

XIONG Guo-liang,YU Ji,ZHANG Hui-hui,etal.Department of Clinical Laboratory,Jiangxi Provincial Chest Hospital.Nanchang 330006,China  
Objective The aim of the study is to employe PCR DNA sequencing to rapidly detect rpoB,KatG gene mutation,and to evaluate its clinical value in the detection of M.tuberculosis (MTB)multi-resistance.Methods The mutations of rpoB and KatG gene were detected with PCRDNA sequencing for 47 RFP-and INH resistant MTB isolates and 30 RFP-and INH-sensitive ones. Results Among 47 multidrug-resistant MTB isolates,43 isolates had rpoB gene mutation,and the mutation rate was 91.5%(43/47);31 isolates had KatG gene mutation,and the mutation rate was 66.0%(31/47);31 isolates had both rpoB and KatG gene mutations,and the mutation rate was 66.0%(31/47).Of 30 drug sensitive MTB isolates,1 strain had KatG gene mutation.Conclusion PCR-DNA sequencing is a seneitive,accurate and specific method for rapid detection of rpoB and KatG gene mutations of MTB,and useful for rapid detection of multidrug resistant MTB.
【Fund】: 江西省卫生厅立项资助项目(20045067)
【CateGory Index】: R440
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