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Study on genetic modes of Rh gene in RHD negative pedigrees

WANG Xiao-zhu,ZHOU Hua-you WU Xu-hua~1,et al.1.Qinhuangdao Blood Center,QinHuangDao 066001,China;2.Nanfang Hospital, Southern Medical University,Guangzhou 510515,China  
Objective To investigate structural feature of RHD gene in Rh negative pedigrees, find out the genetic modes of Rh blood group,and to explore the significance of the detection for RHD gene assortment and Rhesus box in the prediction of hemolytic disease of the newborn.Methods Sequence specific primer PCR(PCR SSP)was applied to detecting the upstream,downstream,hybrid Rhesus boxes and 10 exons of RHD gene in 19 samples from 3 Rh negative pedigrees.Results Only hybrid Rhesus box was detected in individuals with RHD-/RHD-homozygosity;upstream box and downstream box were detected in individuals with RHD +/RHD+ homozygosity;and while upstream, downstream and hybrid boxes were all detected in individuals with RHD+/RHD-heterozygosity. Conclusion It's easy to analyze the structures of RHD gene in pedigrees with PCR-SSP.It is proved that there exists non-expressed RHD gene,which inherit in haplotype mode and accords with Mendelian inheritance's law.
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