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The detection and clinical application of glucose-6-phophate isomerase-containing complex in rheumatoid arthritis

ZONG Ming~1,FAN Lie-ying~1,YANG Lin~1,et al. (1.Department of Clinical Laboratory,Affiliated to Tongji University,Shanghai 200120,China; 2.Department of Clinical Laboratory,Suzhou Hospital of Traditional Chinese Medicine,Suzhou 215002,China)  
Objective To establish a method to measure glucose-6-phophateisomerase(G6PI)-containing complex in serum and synovial fluid of the patients with rheumatoid arthritis(RA) by enzyme-linked immunosorbent assay(ELISA) and to study the mechanism of G6PI/G6PI Ab-containing complex in the development of RA.Methods A Clq antibody capture ELISA was developed to detect Clq/G6PI/G6PI Ab-containing immune complexes.The level of G6PI/G6PI Ab-containing complex in serum of 176 patients with RA,35 with non-RA and 100 healthy donors and 44 synovial fluid of 33 patients with RA and 11 cases with non-RA were tested.Results The values of A450nm of the G6PI/G6PI Ab-containing complex in serum of 176 RA patients,35 non-RA patients and 100 healthy controls were 0.918±0.507,0.746±0.399 and 0.696±0.362 respectively.The level of G6PI/G6PI Abcontaining complex in serum were abnormally increased in RA patients compared with healthy controls(P0.01).Among the RA patients,the active phase and the inactive phase were 1.045±0.529 and 0.764±0.436,respectively.Compared with RA inactive group,they were significantly higher in serum of active group(P0.01).With the values of A_(450 nm) of the healthy control subjects as confidence in terval,the value of the cut—off was 0.755.The sensitivity and specificity of G6PI in the RA patients were 60.2% and 67.4%respectively.The values of A_(450 nm) of the G6PI/G6PI Ab-containing complex in synovia of 33 RA patients and 11 non-RA patients were 0.835±0.497 and 0.596±0.132,respectively.In synovia,the level of G6PI/G6PI Ab-containing complex were significantly higher in RA patients than that in non-RA patients(P0.05).Conclusion The increased expression of G6PI/G6PI Ab-containing complex may play a pathological role in the development of RA,and it may be correlated with the activity of RA.
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