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Investigation of T lymphocyte subsets in fluid of the patients by flow cytometry

MO Yang~1,XU Xiao-dong~2 (1.Department of Clinical Laboratory,Affiliated xiangfan Hospital,Tongji Medicine College, Huazhong Science and Technology University,Xiangfan,Hubei 441021,China)  
Objective To evaluate the method of flow cytometric analysis of T lymphocyte subsets in bodyfluid of the patients. Methods In ovder to get T lymphocytes by 3-colour staining method utilizing SSC/CD3Percp antibody,47 bronchoalveolar lavage fluid and 50 were tested by flow cytometery.Results 1)The percentage of Th is(67.98±7.41)%,Ts(27.36±6.41)%and Th/Ts(2.60±0.64)in BALF with lung cancer,and the percentage of Th is(37.61±4.8)%,Ts(67.97±4.20)%,and Th/Ts(0. 47±0.10)in BALF with lung cancer respectively,and the Th cells in BALF with lung cancer were lower than those in the benign pulmonary disorder(P0.05),and the Th/Ts ratio was significantly lower while the Ts cells were significantly higher in BALF of lung cancer(P0.01),comparied with the benign pulmonary disorder;2)The percentage of Th is(35.76±7.71)%and Ts(64.31±7.37)%and Th/Ts(0.57±0.18) in BALF with lung cancer,and the percentage of Th is(49.27±12.88)%,Ts(44.54±9. 56)%,and Th/Ts is(1.13±0.29) in BALF with benign pulmonary disorder.The Th cells in BALF with lung cancer was lower than those in the benign pulmonary disorder(P0.05),the Th/Ts ratio were significantly lower and the Ts cells were significantly higher in BALF of lung cancer(P0.01) comparied with the benign pulmonary disorder;3)The percentage of This(35.76±7. 71)%,Tsis(64.31±7.37)%and Th/Ts are(0.57±0.18)in BALF with lung cancer,and the percentage of Th is(49.27±12. 88)%,Ts is(44.54±9.56)%,and Th/Ts is(1.13±0.29)in BALF with benign pulmonary disorder.Respectively,the Th cells in BALF with lung cancer were lower than those in the benign pulmonary disorder(P0.05),the Th/Ts ratio were significantly lower and the Ts cells were significantly higher in BALF of lung cancer(P0.01)comparied with the benign pulmonary disorder.Conclusion The immunity function in the patients with lung cancer was significantly decreased.Flow cytometric approach is valuable in analyzing T lymphocyte subsets in BALF.
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