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Drug resistance analysis of methicillin-resistant Staphylococcus aureus positive with panton-valetine leukocidin gene

Wang Fengling1,Zhang Jinyan2,Li Hongyan1,Liu Jing3 (1.Cangzhou Medical College,Cangzhou,Hebei 061001,China;2.Department of Clinical Laboraotory, The Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China;3.Department of Clinical Laboraotory, Cangzhou Hospital of Traditional Chinese and Western Medicine,Cangzhou,Hebei 061001,China)  
Objective To study the resistance of panton-valetine leukocidin(PVL) positive methicillin-resistant Staphylococcus aureus(MRSA) for improving clinical therapy.Methods 47 strains of MRSA were isolated from various clinical specimens.The susceptibility of the MRSA strains to 14 kinds of antibiotics was tested by agar diffusion method.PVL gene was detected by polymerase chain reaction.Results Among all 47 strains of MRSA,the positive rate of PVL gene was 46.8%.The resistant rate of all MRSA strains to β-lactamase antibiotics was up to 100.0%.The resistant rate to aminoglycosides,rifampicin,tetracycline,erythromycin,ciprofloxacin,imipenem of MRSA positive with PVL gene were all up to 100.0%,and to clindamycin was 95.5%.Although antibiotic resistance of MRSA negative with PVL gene was lighter than MRSA positive with PVL gene,the resistant rate also exceeded 88.0% and was not significantly different with MRSA positive with PVL gene.The resistant rate of MRSA to trimethoprim/sulfa exceeded 90.0%.No vancomycin resistant isolate was found.Conclusion The detection rate of PVL gene positive MRAS was relatively high and PVL gene positive MRSA strains might be seriously resistant to antibiotics with multidrug resistance,causing clinical treatment more difficult.
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