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Research on staining technology for the examination of cryptosporidium in the feces of patients with AIDS

Hua Wenhao1,Wang Huizhu1,Jiao Bingxin1,Zhao Hui1,Li Min1,Zhang Yan1,Li Xingwang2 (1.Department of Clinical Laboratory;2.Department of Infectious Disease,Beijing Ditan Hospital,Capital Medical Uniwersity,Beijing 100015,China)  
Objective To explore the staining technology for the examination of cryptosporidium in order to provide laboratory references for the diagnosis of cryptosporidiosis caused by opportunistic infection in AIDS patients.Methods Feces samples of 253 cases of AIDS patients were collected and determined for cryptosporidium by enrich with formalin-ethyl acetate sedimentation and modified acid-fast staining.Results 32 samples were detected with positive result,and the positive rate in the 253 cases of AIDS patients was 12.65%.Conclusion Staining technology,applying formalin-ethyl acetate sedimentation and modified acid-fast staining were more sensitive and efficient,which could be suitable for the application in elementary hospitals.
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