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《International Journal of Laboratory Medicine》 2015-02
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Evaluation on the three methods of Mycobacterium tuberculosis detection

Gu Liping;Lu Weifang;Jiang Wei;Zhao Xiaojue;Department of Clinical Laboratory,Zhangjiagang First People′s Hospital;Center for Disease Control and Prevention in Zhangjiagang;  
Objective To evaluate the clinical value of centrifugal precipitation,Roche culture method and polymerase chain reaction(PCR)for the detection of Mycobacterium tuberculosis.Methods One hundred and two sputum specimens were collected from patients with tuberculosis who had been treated for two months from June 2012 to May 2013.Those specimens were used to detected for Mycobacterium tuberculosis by three methods,and the results from three methods were compared.Results In the 102 samples,the numbers of positive result for Mycobacterium tuberculosis detection were 92(90.19%),48(47.06%)and 77(75.49%)with centrifugal precipitation,Roche culture method and PCR respectively.The coincidence rate of results by precipitation and culture method was 56.86%,81.37% by precipitation method and PCR,and 61.76% by PCR and culture method.Conclusion Combination of the three methods could improve the accurate rate.
【Fund】: 国家科技重大专题项目(2013ZX10004905-002)
【CateGory Index】: R446.5
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