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《International Journal of Laboratory Medicine》 2015-02
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Study on testing T cell subsets by pratical McAb-A-E with quantized control in operational process

Yao Bocheng;Pang Jie;Wang Fang;Tian Caiping;Li Yonghui;Wei Min;Mao Aihong;Bao Xiaoling;Molecular Biology Research Center,Medical Science Institute of Gansu;Department of Clinical Laboratory,Gansu Cancer Hospital;Translation Medical Center,Medical Science Institute of Gansu;Endoscopes Center,Gansu Cancer Hospital;Department of Internal Medicine,Gansu Cancer Hospital;  
Objective To quantize key process of direct McAb-A-E including gently shake,cell smear making and cell count,which would make some steps easier and result exactly.Methods A total of 23 samples were detected lymphocyte antigen levels of CD3+,CD4+,CD8+by the original method and quantized method.In the quantized method,gently shake was replaced by pipettor,cell smear was made by quantized cell suspension,and cell count region was operated with standard.Results There were no significant differences on the level of CD3+,CD4+,CD8+,and the ratio of CD4+and CD8+between quantized method and original method(P0.05).Conclusion Quantizing the key process of McAb-A-E including gently shake,cell smear making and cell count could reduce error in test procedures.
【Fund】: 2010年甘肃省自然科学研究基金项目(1010RJZA144)
【CateGory Index】: R446.6
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【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
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