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《International Journal of Laboratory Medicine》 2015-02
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A rapid Kirby-Bauer method in antimicrobial susceptibility test

Wang Hengqiu;Liu Yufeng;Wang Shuang;Hu Zhifang;Shaoguan City Hospital of TCM Department of Clinical Laboratory;Shaoguan City Hospital of TCM Area Three of Intenal Medicine Department;  
Objective To establish a rapid method of Kirby-Bauer(K-B)for antimicrobial susceptibility test(AST).MethodsRapid K-B method was a K-B method observing bacteriostatic ring diameter at low magnification.K-B and K-B method were used to detect the bacteriostatic ring diameter at different time point.The average checkout time of Staphylococcus aureus,other Staphylococcus aureus,Escherichia coli,other Escherichia coli,Pseudomonas aeruginosa and other bacteria fermentation,the detection rate of of bacteriostatic ring diameter at different observation time,the coincidence rates of standard K-B method,and rapid K-B method were compared.Results The average checkout time of two methods on 6kinds of bacteria bacteriostatic ring diameter(≤48h and ≥72h)had statistically significant differences(P0.05).The average checkout time of the bacteria younger than 48 hours were significant higher than those of bacteria older than 72h(P0.05).The average checkout time of the different bacteria with age younger than 48 hby same method had significant difference(P0.05).The average checkout time of the different bacteria with age older than 72 hby same method had no significant difference(P0.05).Compared with the standard K-B method,the coincidence rate of rapid K-B method was 88.89%-100.00%,that of K-B method was 100.00%.Conclusion Rapid K-B method was faster on obtaining the AST than the standard K-B method and automatic bacteria identification.Standard K-B method for AST,should not wait for 18-24 hto observe bacteriostatic ring diameter,as long as it is visible to the naked eyes could test its diameter and get AST results.
【Fund】: 2014年韶关市科技计划(医学类)项目(2014CX/Ka07)
【CateGory Index】: R446.5
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