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《International Journal of Laboratory Medicine》 2015-02
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Extraction and identification of mouse peritoneal macrophages

Zhang Shuli;Zhang Qi;Jing Xiaohong;Testing Center,Department of Medical Technology,Xi′an Medical University;417 Hospital of Nuclear Industry in Shaanxi Province;  
Objective To explore a simple method for inducing and extracting of mouse peritoneal macrophages.Methods 5% starch broth was used to stimulated inflammatory peritoneal macrophages in mice.Mouse peritoneal macrophages were isolated and cultured in RPMI1640 containing 10% serum.The cell of morphology was observed by microscope.Trypan blue staining was used to identify the cell viability.In vivo phagocytosis of chicken red blood cell phagocytosis rate,phagocytic index determination were detected.Wright′s staining,Immunocytochemical staining were used to identify the cell purity.Results High purity,high activity of macrophages were obtained,which also had the morphological characteristics of macrophages.Conclusion The method in this study is simple and could extract a large number of macrophages.
【Fund】: 西安医学院质量工程检验实验示范中心支持项目(XYZL2010-18);; 西安医学院校级科研基金大学生科研项目(11DXS11)
【CateGory Index】: R446.5
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