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《International Journal of Laboratory Medicine》 2015-02
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Evaluation on automatic BEP-3 enzyme post-processing in enzyme-linked immunosorbent assay

Cai Wangxi;Liu Shiguo;Laboratory of Immunology,Hubei Provincial Hospital of TCM/Hubei University of Chinese Medicine;  
Objective To compare the difference between automatic enzyme-linked immunosorbent assay(ELISA)analysis system and manual processing method.Methods Negative anti-HIV serum samples(240samples)and negative anti-TP serum samples(240samples)were collected randomly in Hubei Provincial Hospital of TCM in 2013.A set of anti-HIV serum plate produced in Zhuhai Lizhu company,a set of anti-HIV standard material produced in Beijing Kangchesitan company,a set of anti-TP serum plate produced in Zhuhai Lizhu Company,and a set of anti-TP standard material produced in Beijing Kangchesitan Company were tested by the BEP-3ELISA analysis system and manual processing method.The absorbance values were measured,and calculated the coefficient of variation(CV)of the two methods,coincidence rate,specificity.Software SPSS 19.0was used to compare the differences of the two methods.Results The detection CVof anti-HIV standard material and anti-TP standard material by manual processing method and BEP-3ELISA analysis system were 12.5%,7.4%,and 13.8%,6.5%.The CVof BEP-3ELISA analysis system was significant lower than that of manual processing method(P0.05).The coincidence rate of the two methods on the positive and negative references of anti-HIV serum plate and anti-TP serum plate were all 100.0%,the lowest detection of the anti-HIV and anti-TP serial dilution products were all 66.7%,77.5%respectively.The specificity of the two methods on the 240 negative samples were 100.0%Conclusion Compared with manual operation,automatic ELISA analysis system has better repeatability,while has same coincidence rate and specificity so the BEP-3ELISA analysis system could replace manual handling for routine clinical sample testing.
【CateGory Index】: R446.6
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1 LI Wensheng1 ZHOU Wei1 LIU Xiaoqin1 ZHOU Shushu2 1.Department of Laboratory Medicine,West China Second University Hospital,Sichuan University,Chengdu 610041; 2.Sichuan university Hospital wangjiang Division,Chengdu 610021,China;Comparative Study of ELISA Automatic Analyzer and ELISA Semi-automatic Microplate Reader[J];China Foreign Medical Treatment;2011-24
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