Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Paeoniflorin suppresses the maturation and function of bone marrow-derived dendritic cells

ZHANG Han;LUO Qingqiong;ZHU Liping;DING Jieying;CHEN Fuxiang;Department of Clinical Laboratory,Shanghai Ninth People′s Hospital,Shanghai Jiao Tong University School of Medicine;  
Objective To explore the effect of paeoniflorin on the phenotype and function of dendritic cells(DC).Methods Bone marrow cells isolated from C57 BL/6 mice were stimulated with granulocyte-macrophage-colony stimulating factor(GM-CSF)and interleukin-4(IL-4)to induce bone marrow-derived dendritic cells,and paeoniflorin of different concentrations was added into the culture system.The cell counting kit-8(CCK-8)assay was used to detect cell viability.The expression levels of co-stimulatory molecules CD80,CD40 and MHCⅡon immature DC were analyzed by flow cytometer.Phagocytosis of immature DC was determined by measuring the cellular uptake of FITC-dextran.Mixed-lymphocyte reaction assay was used to investigate the effect of paeoniflorin on mature DC-dependent induction of allogeneic lymphocyte proliferation.ResultsPaeoniflorin did not affect the cell viabilities and had no obviously toxic effect.paeoniflorin inhibited the expression of CD40 and MHC Ⅱ on immature DC,as well as the phagocytosis of immature DC.Furthermore,paeoniflorin suppressed mature DC-induced allogeneic lymphocyte proliferation.Conclusion Paeoniflorin significantly suppressed the maturation and function of DC,thus our present data may provide experimental evidences for the usage of paeoniflorin in treating autoimmune diseases.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved