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The correlation analysis between syndrome types of traditional Chinese medicine and HLA-DP gene polymorphisms in patients with refractory chronic hepatitis C

HAN Shuxia;ZHENG Yi;PENG Xiaoming;ZHAO Youyun;Department of Clinical Laboratory,Hubei Provincial Hospital of Traditional Chinese Medicine;Hubei Province Academy of Traditional Chinese Medicine;  
Objective To investigate the correlation between syndrome types of traditional Chinese medicine(TCM)and HLA-DP gene polymorphisms in patients with refractory chronic hepatitis C(RCHC),and to find suitable molecular markers for TCM syndromes and provide scientific basis for TCM syndrome differentiation.Methods A total of 200 RCHC patients of Hubei Provincial Hospital of Traditional Chinese Medicine were enrolled retrospectively.Their general characteristics,TCM syndromes,data of liver function(ALT,AST,ALP and ALB),HCV RNA,HCV genotypes,PT and PLT were collected.At the same time,100 patients from other departments of the hospital were enrolled as control group.FQ-PCR was used to detect the genotypes of the HLA-DP gene rs9277535 sites of the RCHC patients with different TCM syndromes and the patients of control group.The correlation between the data and TCM syndromes was statistically analyzed.Results Among 200 patients in the study,the pattern of liver-depression and spleen-deficiency was maximum(100 cases,50.0%),while the remaining syndromes were dampness and heat resistance(48 cases,24.0%),yin deficiency of liver and kidney(32 cases,16.0%)and obstruction of collaterals by blood stasis(20 cases,10.0%).For age,data of liver function index and HCV RNA,there was no statistical difference between the liver-depression and spleen-deficiency type and other TCM syndromes(P0.05).But the difference of PT was significant between liver-depression and spleen-deficiency type and other TCM syndromes(P0.05).Compared with control group,there was no significant difference between the HLA-DP gene rs9277535 genotype GG and AA in the liver depression spleen deficiency group and the non-liver depression spleen deficiency group in the RCHC patients(P0.05).Conclusion There was no significant difference between the genotypes of HLA-DP gene rs9277535 genotype GA heterozygous genes and AA homozygous genes in the different TCM syndromes of RCHC patients.However,the distribution of alleles G and A in liver depression spleen deficiency group and non-liver depression spleen deficiency group showed a different trend,which is worthy of further study.
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