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Distribution of Mycobacterium tuberculosis resistance genes in a hospital in Changsha

PAN Jianhua;SHI Guomin;MA Xiaohua;XIANG Yangen;Changsha Central Hospital;  
Objective To study the distribution of MTB resistance genes in Changsha Central Hospital.Methods Totally 1981 strains of MTB were identified in Changsha Central Hospital from July 2014 to March2017,resistant genes ropB 、KatG and inhA were screened by DNA microarray.Results A total of 335 ropB mutant strains were detected,with a mutation rate of 16.91%(335/1981).Site mutation rates were followed by 531(171/335,50.75%),516(71/335,21.19%),526(57/335,17.01),511(48/335,14.33%),513(9/335,2.69%),and 533(8/335,2.39%);The KatG mutant was detected in 310 strains,the mutation rate was15.65%(310/1981);the inhA mutant was 68,and the mutation rate was 3.43%(68/1981).The total mutation rate of isoniazid resistant genes was 18.6%(369/1981).KatG AGC→ACC mutant accounted for 79.95%(295/369).Conclusion Mutations of the 531,516,526 and 511 loci of ropB gene and mutations of KatG AGC→ACC are the main factors resulting RFP and isoniazid resistance in tuberculosis in Changsha Central Hospital.DNA microarray can be used as a rapid screening method for MTB resistance genes in Changsha.
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