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Preliminary study on detection of hard-type fatty acid binding protein in human serum based on colloidal gold immunological colorimetric assay

SUN Yu;WANG Ru;GU Lilin;LI Jialin;WANG Huizhong;Department of Clinical Laboratory,the 305th Hospital of PLA;  
Objective To establish a colloidal gold colorimetric assay method for detection of hard-type fatty acid binding protein(h-FABP)in serum.Methods According the principle of homogeneous colorimetry for quantitative determination of antigen or antibody in a sample,based on the changes of absorption peak of colloidal gold adsorption marker at 540 and 660 nm,we established a method for detecting h-FABP in serum.Results The colloidal gold particles were well distributed,and the prepared antibodies labeled colloidal gold particles were homogeneous distribution,which were suitable for the requirements of the test.In the preliminary analysis of the clinical specimens,compared with the Immune Turbidimetry method,the coincidence rate was95.09%.Conclusion A colloidal gold colorimetric assay method for h-FABP in the human serum had been established preliminarily,the results were certain consistent with those obtained by Immune Turbidimetry method,and can be further applied to clinical detection.
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