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Appraisal of three plasma creatinine test methodology

WANG Meizhu;KAN Wei;HUANG Qinfeng;CHEN Min;Department of Clinical Laboratory,Fuzhou General Hospital;  
Objective To evaluate the results of plasma creatinine determined by imide Hydrolase method(hereinafter referred to as UV enzymatic method),sarcosine oxidase enzymatic method and Jaffémethod on biochemical Analyzer Cobas 8000 c702.Methods According to the American clinical and laboratory Standards Institute(CLSI)guidelines EP5-A2,EP6-A,C28-A2,EP9-A2,EP7-A2 and other documents,the precisions,linear ranges,reportable ranges,methodological comparisons,drug interferences,reagents stability of three methods for determination of creatinine were evaluated.Results The within-run precision and between-run precision of three methods were less than 1/4 TEa and 1/3 Tea,respectively.The linear ranges of UV enzymatic method,sarcosine oxidase enzymatic method and Jaffémethod were 4-2 017μmol/L,8-1 966.5μmol/L,and3-1 310.5μmol/L respectively,and their reportable ranges were as follows:4-20 160μmol/L,8-51 900μmol/L and 3-10 080μmol/L.The methodological comparison shows that these three above-mentioned methods correlate to each other very well with a correlation coefficient of more than 0.99.However,in interference test,we found that the sarcosine oxidase enzymatic method of creatinine quantification could be affected significantly by the negative interference of the presence of 5.24μg/mL calcium dobesilate or 0.016 g/L etamsylate,but there were no marked difference in the other two methods even under the maximum dose.In the stability of reagent within the instrument after open,the reagents of Jaffémethod without calibration could maintain stability only two days and show obvious decreasing trend,but the reagents were more stable in the other two methods.Conclusion Based on the above data,we presented the view that UV enzyme method is the most reliable in these three above-mentioned methods of detecting creatinine.
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