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Application of AtheNA-Lyte system in the detection of antinuclear antibodies

ZHANG Cheng;WU Shanshan;LV Dan;Department of Clinical Laboratory,Affiliated Hospital of Liaoning University of Traditimal Chinese Medicine;  
Objective In order to screen and detect the autoimmune disease,a multiplexed liquichip assay for combined ANAs was evaluated.Methods 210 serum samples were tested utilizing the AtheNA system and LIA method:120 samples from patients with autoimmune disease(case group),90 samples from healthy donors(healthy ontrol group).The antinuclear autoantibodies to 15 different analytes(Ro52,SSA,SSB,Sm,RNP,Scl-70,Jo-1,Rib,His,PCNA,CB,PM,M2,NUC,dsDNA)were tested.Sensitivity,specificity,cross-reactivity and application were identified by AtheNA system.Results There were no significant difference in the antinuclear antibodies positive rate between two groups(P0.05).Antinuclear autoantibodies screening by AtheNA system revealed high concordance with the LIA in samples without cross-reactivity(P0.05).Conclusion The AtheNA system showed sensitivity and specificity for simultaneous quantitative detection of combined antinuclear antibodies.
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