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The application and progress of LAMP in clinical microorganism detection

GUO Qi;YANG Suqing;Chongqing Technical Center of Drug Evaluation Certification;Chongqing Quality Testing & Inspection Center of Medical Devices;  
Loop mediated Isothermal Amplification(LAMP)is a technique for amplification of target DNA sequences under isothermal conditions.It is a hot research topic in recent years.In clinical microorganism detection,Compared with staining microscopy,biochemical identification,reverse transcriptase chain reaction(RT-PCR)and nuclear magnetic resonance(NMR),LAMP has the advantages of simplicity,strong specificity,easy observation and no need of precision instruments.It is a very valuable detection technology.In this paper,the end point detection method of LAMP and the application and progress of LAMP in clinical microorganism detection are reviewed,and the advantages and disadvantages of the application are analyzed.The purpose of this article is to provide a reference for examiners to select lamp as a means of clinical microorganism detection.
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