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《International Journal of Laboratory Medicine》 2019-10
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Silencing EZH2 gene inhibits proliferation of gastric cancer cell and promotes its apoptosis

YANG Biwei;ZHAO Erchuan;SHI Xiang;LIU Qiang;Department of Clinical Laboratory,Guizhou Provincial People′s Hospital;Department of Clinical Laboratory,Yuncheng First Hospital;Department of Pathology,Qianjiang Central Hospital;  
Objective To explore the effect of siliencing EZH2 on proliferation and apoptosis in gastric cancer cell line MGC803.Methods Silencing EZH2 was performed by siRNA transfection,qRT-PCR and Western blot were used to verify the efficienly of silence.The effect of proliferation and apoptosis were detected by MTT assay and flow cytometry,respectively.Western blot was used to indicate the expression of Bcl-2 and Bax.Results EZH2 gene was successfully silenced in gastric cancer cells.The proliferation of silencing group was significantly lower than that of normal group and negative control group,the apoptosis rate was significantly increased compared with the normal group and negative control group,while the protein expression of Bcl-2 in siRNA group was significantly down-regulated compared with the control and negative control group but Bax was the opposite with statistically significant difference(P0.05).Conclusion Silencing EZH2 gene can inhibit proliferation and promotes apoptosis in gastric cancer cells.
【Fund】: 贵州省科学技术厅项目(黔科合LH字[2014]7008)
【CateGory Index】: R735.2
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