Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《International Journal of Laboratory Medicine》 2019-10
Add to Favorite Get Latest Update

Effect of down-regulating FBXO22 gene on invasion and migration in breast cancer cells

FANG Hongshu;ZHANG Wei;Department of Clinical Laboratory,Chongqing Fourth People′s Hospital;  
Objective To explore the effects of FBXO22 gene silencing on the invasion and migration ability of MDA-MB-231 cells in breast cancer and investigate the related molecular mechanisms.Methods FBXO22 small fragments interference RNA were used to transfect breast cancer MDA-MB-231 cells for down-regulating FBXO22 gene expression,and the cell transfection efficiency was detected by Western blot,the effect of down-regulating FBXO22 gene expression on cell invasion ability in breast cancer by cell scratch test,the effect of down-regulating FBXO22 gene expression on cell migration ability in breast cancer by Transwell Chambers experiment,the effect of down-regulating of FBXO22 gene expression on invasion and migration related protein expression in breast cancer cell by Western blot.Results After transfection of FBXO22-siRNA,the expression of FBXO22 in breast cancer MDA-MB-231 cells was significantly decreased,the ability of migration and invasion were reduced,the level of E-cadherin was increased,and the level of Vimentin,MMP-2 and MMP-9 was decreased.Conclusion The down-regulation of FBXO22 can inhibit migration and invasion of breast cancer cells.The underlying mechanism may be that the down-regulation of FBXO22 gene expression blocks the epithelial-mesenchymal transformation process of MDA-MB-231 cells and reduces the expression of related to cell metastasis proteins MMP-2 and MMP-9.
【CateGory Index】: R737.9
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved