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《International Journal of Laboratory Medicine》 2019-10
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Influence of high concentration of triglyceride in detecting with turbidimetric inhibition immunoassay

SONG Lin;DAI Wen;ZHANG Ning;Department of Clinical Laboratory,Renmin Hospital of Wuhan University;Department of Nephrology,Renmin Hospital of Wuhan University;  
Objective To analyze the influence of different concentrations of triglyceride in detecting items with turbidimetric inhibition immunoassay,using the fat emulsion to imitate the lipemia samples.MethodsFive lipemia samples(M1 was with 0μL fat emulsion and 20μL normal saline,M2 was with 5μL fat emulsion and 15μL normal saline,M3 was with 10μL fat emulsion and 10μL normal saline,M4 was with 15μL fat emulsion and 5μL normal saline,M5 was with 20μL fat emulsion and 0μL normal saline),with different concentrations of triglyceride were imitated by adding different volume of fat emulsion into basic serum.Automatic biochemical analyzer was used to detect the test items with turbidimetric inhibition immunoassay in different group,then to detect the values of test items with turbidimetric inhibition immunoassay through high speep centrifugal(12 000 r/min for 10 min)before and after the centrifugal.Results With the increasing of the concentration of triglyceride,the detection value of Fn was increasing and the detection value of retinol-binding protein(RBP)was decreasing among the 5 groups with statistically significant difference(P0.05).While the concentration of triglyceride9 mmol/L,the level of Prealbumin(PA)was decreasing with the increasing of triglyceride among the M3 group,M4 group and M5 group,with statistically significant difference(P0.05).Compare with the M1 group,the level of complement C1 q in the M2 group,M3 group,M4 group and M5 group showed statistically significant difference(P0.05);compared with M5,the level of complement C1 q in the M2 group,M3 group and M4 group showed statistically significant difference(P0.05).The difference among the 5 groups on Cysstatin C(CYC),β2-microglobulin(β2-MG),Lipoprotein a(Lp(a)),apolipoprotein A1(ApoA1),apolipoprotein B(ApoB)and wide range high sensitive C-reaction protein(wr-CRP)had no difference(P0.05).The level of triglyceride in the M2 group,the levels of TG,Fn and RBP in the M3 group and the levels of TG,Fn,RBP and PA in the M4 group and the M5 group before and after ultracentrifugation showed statistically significant difference(P0.05).Conclusion Different concentrations of triglyceride may have different influence on different test items with turbidimetric inhibition immunoassay item,although ultracentrifugation could reduce the impact,it can not eliminate completely.Technicians and physicians should be aware of the influence that lipemia can have on the results of the laboratory tests,in order to reduce errors,better interpretation of the report and increase patient safety.
【CateGory Index】: R446.6
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