Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《International Journal of Laboratory Medicine》 2019-10
Add to Favorite Get Latest Update

Influence of high concentration of triglyceride in detecting with turbidimetric inhibition immunoassay

SONG Lin;DAI Wen;ZHANG Ning;Department of Clinical Laboratory,Renmin Hospital of Wuhan University;Department of Nephrology,Renmin Hospital of Wuhan University;  
Objective To analyze the influence of different concentrations of triglyceride in detecting items with turbidimetric inhibition immunoassay,using the fat emulsion to imitate the lipemia samples.MethodsFive lipemia samples(M1 was with 0μL fat emulsion and 20μL normal saline,M2 was with 5μL fat emulsion and 15μL normal saline,M3 was with 10μL fat emulsion and 10μL normal saline,M4 was with 15μL fat emulsion and 5μL normal saline,M5 was with 20μL fat emulsion and 0μL normal saline),with different concentrations of triglyceride were imitated by adding different volume of fat emulsion into basic serum.Automatic biochemical analyzer was used to detect the test items with turbidimetric inhibition immunoassay in different group,then to detect the values of test items with turbidimetric inhibition immunoassay through high speep centrifugal(12 000 r/min for 10 min)before and after the centrifugal.Results With the increasing of the concentration of triglyceride,the detection value of Fn was increasing and the detection value of retinol-binding protein(RBP)was decreasing among the 5 groups with statistically significant difference(P0.05).While the concentration of triglyceride9 mmol/L,the level of Prealbumin(PA)was decreasing with the increasing of triglyceride among the M3 group,M4 group and M5 group,with statistically significant difference(P0.05).Compare with the M1 group,the level of complement C1 q in the M2 group,M3 group,M4 group and M5 group showed statistically significant difference(P0.05);compared with M5,the level of complement C1 q in the M2 group,M3 group and M4 group showed statistically significant difference(P0.05).The difference among the 5 groups on Cysstatin C(CYC),β2-microglobulin(β2-MG),Lipoprotein a(Lp(a)),apolipoprotein A1(ApoA1),apolipoprotein B(ApoB)and wide range high sensitive C-reaction protein(wr-CRP)had no difference(P0.05).The level of triglyceride in the M2 group,the levels of TG,Fn and RBP in the M3 group and the levels of TG,Fn,RBP and PA in the M4 group and the M5 group before and after ultracentrifugation showed statistically significant difference(P0.05).Conclusion Different concentrations of triglyceride may have different influence on different test items with turbidimetric inhibition immunoassay item,although ultracentrifugation could reduce the impact,it can not eliminate completely.Technicians and physicians should be aware of the influence that lipemia can have on the results of the laboratory tests,in order to reduce errors,better interpretation of the report and increase patient safety.
【CateGory Index】: R446.6
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 WANG Jian-zhu;ZHOU Ju-he;JIA Xing-wang;DONG Zhen-nan;TIAN Ya-ping;Department of Clinical Biochemistry,PLA General Hospital;;Method Evaluation on Serum Concentration of Anti-treponema Pallidum Antibody by Immunoturbidimetry[J];标记免疫分析与临床;2015-01
2 LI Jianfen The Fire Corps Clinic of Hebei Province, Shijiazhuang 050035, China;Cause analysis about unqualified blood samples on clinical inspection[J];中国当代医药;2012-27
3 Yan Renqing 1,Gao Song2 (1.Clinical Laboratory Center of Affiliated Hospital,Zunyi Medical College,Zunyi Guizhou 563000,China;2.Department of Laboratory Medicine,Zunyi Medical College,Zunyi Guizhou 563000,China);Comparison of interference methods in elimination of blood lipids with clinical biochemistry examination of common indexes[J];遵义医学院学报;2011-06
【Co-citations】
Chinese Journal Full-text Database 8 Hits
1 XU Jian’guo;Xinxian The People's Hospital;;Analysis the Measures of Common Index Interference and Elimination Lipoidemia in Clinical Biochemical Detection[J];中国继续医学教育;2015-29
2 Xu Xiao-lin;The people's hospital of Baoying county,Jiangsu;;To compare the effect of the interference of the blood lipid in the biochemical test results by comparing the dilution method with the high speed centrifugal method[J];世界最新医学信息文摘;2015-79
3 ZHANG Yan-xia;YU Yan-hua;Community health service center of Binzhou City;;Reason analysis of unqualified blood samples in clinical laboratory[J];中西医结合心血管病电子杂志;2015-27
4 Xu Mei;Yan Renqing;the Department of Medical Laboratory,Zunyi Medical University;Department of Medical Laboratory,The Affiliated Hospital of Zunyi Medical University;;The patient data moving average method are applied to no quality product project and batch quality control between analytical phase of clinical biochemistry[J];遵义医学院学报;2015-04
5 TAO Lin-jing;Nanyang Central Hospital;;Research on Reasons of Unqualified Blood Specimens in Clinical Test and Corresponding Counter measures[J];中国医学创新;2015-02
6 WANG Yan;XU Lingling;YANG Mei;Department of Clinical Laboratory, The Affiliated Hospital of Luzhou Medical College;;Analysis of the Causes and Countermeasures of Clinical Inspection Unqualified Blood Specimens[J];中外医疗;2014-05
7 LENG Wei;Jilin Taonan City Hospital;;Analysis of the Importance of Blood Samples Which Are Qualified or Not on the Test Results[J];中外医疗;2014-03
8 QIAN Jian-ping;XIONG Huai-min;JIANG Ting-wang;Changshu City Medical Inspection;;Comparison of Methods for Eliminating Blood Lipid Disturbance to the Detection of Clinical Biochemical Indexes[J];北华大学学报(自然科学版);2013-05
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 QU Gui-qing.Xixiang People's Hospital Baoan District,Shenzhen 518102,China;The Analysis of Causes of 69 Samples for Failure Analysis/[J];中外医学研究;2012-12
2 XUE Daqi Research Center of Sexology,Peking University Health Science Center,Beijing 100191;Challenges and countermeasures for syphilis prevention and treatment[J];中国性科学;2012-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved