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《Guangdong Trace Elements Science》 2007-01
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Determination of Trace Lead by DNA Electrochemical Biosensor

GAN Ning,WANG Zhiying,XU Weimin,LI Tianhua (Faculty of Material Science and Chemical Engineering,Ningbo University,Ningbo 315211,China)  
One singe strand DNA modified electrode was prepared by physical absorption method.The electrode was employed to determine Pb in real waters in 0.2 mol/L NaAc-HAc solution(pH 5),lead ion was accumulated to form Pb-DNA complex on the chemically modified electrode.A sensitive oxidation peak of Pb(2+) was observed with differential pulse voltammetry(DPV) method.The peak potential is 0.15 V(vs SCE).The relationship current between and concentration is linear from 5.0×10(-8) to 1.0×10(-6) mol/L.The detection limit is 2.0×10(-8) mol/L.
【Fund】: 浙江省科技攻关项目(No.2006C31040);; 浙江省教育厅科研项目(No.20061697);; 宁波市自然科学基金(No.2006A610048);; 宁波大学学生创新基金资助项目
【CateGory Index】: O661.1;O657.1
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