Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《International Journal of Genetics》 2006-01
Add to Favorite Get Latest Update

Secretoglobin-3A1 Gene Promoter Hypermethylation Relevant to Esophageal Squamous Cell Carcinoma

LIU Shuang~1,GUO Ming-zhou~2,LI Jian-sheng~2,DUAN Fang-ling~3,LU Feng-min~4.(~1Osaka Beijing Research Institute,Beijing 100027;~2The First Teaching Hospital,Zhengzhou University,Zhengzhou 450052;~3Institute of Gastroenterology of Zhengzhou University,Zhengzhou 450014; ~4Department of the Microbiology,Peking University of Health Science Center, Beijing 100083,P.R.China)Corresponding author: LU Feng-min.E-mail: lufengmin@bjmu.edu.cn  
Objective To study the involvement of promoter region hypermethylation related loss of gene expression of Secretoglobin-3A1(SCGB3A1),a cytokine with tumor suppressing activity,in the tumorigenesis of oesophageal squmous cell carcinoma(ESCC).Methods RT-PCR was used to detect SCGB3A1 expression in 12 cell lines derived from human ESCC;methylation specific PCR(MSP) was used to test the methylation statue of SCGB3A1(gene's) promoter region CpG island in those ESCC cell lines and 37 primary ESCC specimen from Henan China.DNA de-methylation regent 5-aza-dc was also used to treat the SCGB3A1 silencing cell line in vitro.Results Only Kyse410,Kyse520 and TE8 from 12 tested cell lines had detectable SCGB3A1 gene expression.As revealed by using MSP,SCGB3A1 gene silencing was associated with the hypermethylation state throughout its promoter region,which was further confirmed by the detection of SCGB3A1 gene re-expression in Kyse30,after in vitro de-methylation treatment with 5-aza-dc.Moreover,we found that 18/37(48.6%) of primary ESCC specimens tested had a high density of methylated CpG in SCGB3A1 promoter region.Conclusion Data here suggest promoter-hypermethylation related loss of SCGB3A1 gene expression is relevant to ESCC.
【Fund】: 国家自然科学基金主任基金(No.30540081)
【CateGory Index】: R735.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 DU Ji-mei , LI Xiao-ming, GUO Ming-zhou , SHI Shuang, LI Jie, ZHUANG Hui , LU Feng-min. ( Department of Laboratory Medicine, Wenzhou Medical College, Wenzhou 325023 ;Nanyang Higher Medical College, Nanyang 473058; Department of Gaslroenterology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052; Department of Microbiology, Peking University of Health Science Center, Beijing 100083; P. R. China);Abnormal Hypermethylation of ERαPromoter Involved in Esophageal Squamous Cell Carcinoma[J];International Journal of Genetics;2006-06
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Lei, XING De yin, TAN Wen, HE Zu gen,LIN Dong xin* Cancer Institute & Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, P.R.China;Promoter Hypermethylation of DNA Repair Gene MGMT in Esophageal Squamous Cell Carcinoma[J];Chinese Journal of Cancer;2001-12
2 ZHANG Song-fa, YE Feng, CHEN Huai-zeng, XIE Xing. (Women's Hospital School of Medicine Zhejiang University, Hangzhou 310006, P.R.China);Advance in the Profile of Genome CpG Islands Methylation Methods[J];International Journal of Genetics;2006-03
3 GUO Xiao-qing1, WANG Shi-jie1, ZHANG Jian-hui2, CONG Qing-wen1, ZHANG Li-wei1,GUO Wei2,WANG Xiao-ling1, KUANG Gang21. Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, P.R.China2. Department of Molecular Biology, Hebei Cancer Institution, Shijiazhuang 050011, P.R.China;CpG island methylation of p16 gene in tissues of esophageal squamous cell carcinoma andits precancerous lesions[J];Journal of Qilu Oncology;2005-07
4 TIAN Zi-Qiang,LIU Jun-Feng,LI Yong,et al. Department of Thoracic Surgery,Fourth Hospital of Hebei Medical University,Shijiazhuang,050011;Significance of CpG Island Methylation of MT-3 gene in Cell Lines of Esophageal Cancer[J];The Practical Journal of Cancer;2005-02
5 Mingzhou Guo,M .D .,Fh .D,Michael G.House,M,D .,Yoshimitsu Akiyama,Ph .D.Yu Qi,M .D,et al Division of Tumor Biology ,Department,of Oncology ,The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins ,Baltimore MD ,China;Hypermethylation of the GATA gene family in esophgeal cancer[J];Chinese Journal of Gastroenterology and Hepatology;2003-02
6 Zhao Xuexin, Shen Wangzhen,Wang Lirong,et alDepartment of Biochemistry,Xinjiang Medical College;Loss of Heterozygosity at Chromosomes 3p24 Allelic in the Kazak Patients with Esophageal Cancer[J];Journal of Xinjiang Medical University;1996-03
7 Kurash, Wang Lirong, Zhao Xuexin, et al Department of Biochemistry, Xinjiang Medical University;Study on the Loss of Heterozygosity and Point Mutation of APC Tumor Suuppressor Gene in Xinjiang Kazak's Esophageal Cancer[J];JOURNAL OF XINJIANG MEDICAL UNIVERSITY;1999-02
8 Shen Wangzhen, Zhao Xuexin, Wang Lirong, et al Department of Biochemistry, Xinjiang Medical University;A Study on the Ras Oncogene Mutation in Esophageal Cancer of Xinjiang District[J];JOURNAL OF XINJIANG MEDICAL UNIVERSITY;1999-04
9 Kurash, WANG Li-rong, ZHAO Xue-xin, et al (Department of Biochemistry, Xinjiang Medical University);Study on the homozygous deletion and expression of the tumor suppressor gene p16 in Xinjiang Kazak's esophageal cancer[J];JOURNAL OF XINJIANG MEDICAL UNIVERSITY;2000-01
10 CHEN Jian, WANG Bing yu, Scander,(Department of Biochemistry , Xinjiang Medical University);Putative tumor suppressor genes on chromosome 3p24 and 11p15.5 in esophageal carcinoma[J];JOURNAL OF XINJIANG MEDICAL UNIVERSITY;2000-03
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 Ding Shigang,Wu Di,Zou Jianping,et al.Department of Digestive Diseases,Peking University Third Hospital,Beijing 100083,China;Hypermethylation Status of RASSF1A Gene Promoter Region in Esophageal Squamous Cell Carcinoma[J];Chinese Journal of Minimally Invasive Surgery;2007-12
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved