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《International Journal of Genetics》 2006-01
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Secretoglobin-3A1 Gene Promoter Hypermethylation Relevant to Esophageal Squamous Cell Carcinoma

LIU Shuang~1,GUO Ming-zhou~2,LI Jian-sheng~2,DUAN Fang-ling~3,LU Feng-min~4.(~1Osaka Beijing Research Institute,Beijing 100027;~2The First Teaching Hospital,Zhengzhou University,Zhengzhou 450052;~3Institute of Gastroenterology of Zhengzhou University,Zhengzhou 450014; ~4Department of the Microbiology,Peking University of Health Science Center, Beijing 100083,P.R.China)Corresponding author: LU Feng-min.E-mail: lufengmin@bjmu.edu.cn  
Objective To study the involvement of promoter region hypermethylation related loss of gene expression of Secretoglobin-3A1(SCGB3A1),a cytokine with tumor suppressing activity,in the tumorigenesis of oesophageal squmous cell carcinoma(ESCC).Methods RT-PCR was used to detect SCGB3A1 expression in 12 cell lines derived from human ESCC;methylation specific PCR(MSP) was used to test the methylation statue of SCGB3A1(gene's) promoter region CpG island in those ESCC cell lines and 37 primary ESCC specimen from Henan China.DNA de-methylation regent 5-aza-dc was also used to treat the SCGB3A1 silencing cell line in vitro.Results Only Kyse410,Kyse520 and TE8 from 12 tested cell lines had detectable SCGB3A1 gene expression.As revealed by using MSP,SCGB3A1 gene silencing was associated with the hypermethylation state throughout its promoter region,which was further confirmed by the detection of SCGB3A1 gene re-expression in Kyse30,after in vitro de-methylation treatment with 5-aza-dc.Moreover,we found that 18/37(48.6%) of primary ESCC specimens tested had a high density of methylated CpG in SCGB3A1 promoter region.Conclusion Data here suggest promoter-hypermethylation related loss of SCGB3A1 gene expression is relevant to ESCC.
【Fund】: 国家自然科学基金主任基金(No.30540081)
【CateGory Index】: R735.1
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