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《International Journal of Genetics》 2006-01
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An Analysis of Single Nucleotide Polymorphism on HBV DNA U5 Sequence of Hepatitis B Patients and Their Children

WANG Pei-lin~(1,2),WANG Xiu-hai~2,CONG Shu-ying~2,MA Hong-ming~1,ZHANG Xue-cheng~1.(~1College of Maring Life Science,Ocean University of China,Qingdao 266003;~2Medical College,Qingdao University,Qingdao 266021,P.R.China)Corresponding author: ZHANG Xue-cheng.E-mail: xczhang@hotmail.com  
Objective To study further vertical transmission of hepatitis B between the hepatitis B patients and their children.Methods 30 families(68cases) of hepatitis B patients and their children were examined by the molecular genetic methods and techniques of single nucleotide polymorphism(SNP) and polymerase chain reaction single strand configuration polymorphism(PCR-SSCP).Results It showed that the frequency of freed and integrated HBV DNA were higher consistently between the hepatitis B patients and their children born after the occurrence of hepatitis B,and they had more significant differences than the children born before their parent suffered from hepatitis B,P0.05.SNPs of hepatitis B patients and their children were ananlysed and discovered that there were base substitution,insertion or deletion in many base sites of HBV DNA U5 sequence and non-U5 sequence,(1 908 A→T),(1 950 G→T),(1 967 T→C),and deletion in 1 900T,insertion in(1 903C) and so on.The SNPs in the loci of(1 908)、(1 950)、(1 967)、(1 900) and (1 903) of male hepatitis B patients and their children born after the occurrence of hepatitis B was identical.Conclusion Hepatitis B may be inherited from the male hepatitis B patients to their children born after the occurrence of hepatitis B;thereby,a molecular genetic evidence is provided further for hereditary transmission of hepatitis B.
【Fund】: 国家自然科学基金(No.3870310);; 山东省自然科学基金(No.91C0125)
【CateGory Index】: R512.62
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