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《International Journal of Genetics》 2006-01
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Construction and Identification of Mouse H_2B-GFP Eukaryotic Expressing Vector

SUN Wen-jing~1,MA Lin-lin~1,HAN Fei-fei~1,REN Cong~1,FU Song-bin~(1,2).(~1Laboratory of Medical Genetics,Harbin Medical University, Harbin 150081;~2Bio-pharmaceutical Key Laboratory of Heilongjiang Province,Harbin 150081,P.R.China)Corresponding author: FU Song-bin.E-mail: fusb@ems.hrbmu.edu.cn  
Objective To construct a fluorescent eukaryotic expression vector coding mouse H_2B gene.Methods The mouse H_2B cDNA was amplified by RT-PCR from the total RNA and inserted into pcDNA3.1/CT-GFPTOPO vector.The structure of the recombinant vector had been verified by PCR amplification,restriction(analysis) and sequencing.Then the reporting vector was transfected into mouse fibroblast cell line NIH3T3.Results The recombinant fluorescent expression vector coding mouse H_2B gene was correctly constructed in size,orientation and sequence,which was expressed in the nucleus of NIH3T3.Conclusion The recombinant mouse H_2B-GFP expression vector has been successfully constructed,which can help study the biochemical role of H_2B and chromosomes morphologic variation.
【Fund】: 国家自然科学基金资助项目(No.30370783);; 黑龙江省卫生厅医学科研课题(No.2005-30);; 高等学校优秀青年教师教学科研奖励计划;; 教育部高等学校博士学科点专项科研基金(No.20040226001);; 哈尔滨医科大学研究生创新基金资助项目
【CateGory Index】: R346
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Chinese Journal Full-text Database 1 Hits
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