Protection of flavonoid components baicalein,quercetin,or sodium danshensu on cytotoxicity and cellular DNA damage induced by cigarette smoke

CHEN Hong-jun;GAN Zhi-bin;LIU Xia;YU Zi-cheng;FENG Yi-shan;LU Jian-zhong;Institute of Clinical Pharmacy and Pharmacology, Tongji University School of Medicine;Department of Pharmaceutical Analysis, School of Pharmacy, Fudan University;  

Objective To evaluate the protective effect of flavonoid components baicalein, quercetin or sodium danshensu on cytotoxicity and cellular DNA damage induced by cigarette smoke. Methods Two kinds of eukaryotic cells B-16 cells and oral buccal mucosal cells in liquid were attacked by the mainstream smoke produced via on-line cigarette smoking according to FTC with or without baicalein, quercetin or sodium danshensu. The cytotoxicity and cellular DNA damage were respectively assessed by MTT method and single cell gel electrophoresis. Results The cytotoxicity of B-16 cells and DNA damage of two kinds of cells induced by on-line cigarette smoking were obvious. A positive relationship was observed between the exposure time in smoke and the tail moment and olive tail moment of comet cells. Baicalein, quercetin, or sodium danshensu(1 mmol/L) could relieve the cytotoxicity by about 50% and decrease the DNA damage by more than 60%. Conclusion There is a positive relationship between the exposure of cells in smoke and the cellular DNA damage, and the flavonoid components baicalein, quercetin, or sodium danshensu have a protective effect against the genotoxicity of cigarette smoke.

【Fund】： 国家自然科学基金资助项目(81302741)
【CateGory Index】： R285.5

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