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《Journal of Lanzhou University of Arts and Science(Natural Science Edition)》 2019-03
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Extraction of Polysaccharide from Agaricus Blazei Murrill and Its Protective Effect on Nerve Cell Injury

LI Hong-xia;WU Chao;WANG Qing;School of Biology and Food Engineering, Suzhou University;  
The aim of this study is to optimize the extraction process of polysaccharides from agaricus blazei murrill and the protective effect of polysaccharides from agaricus blazei murrill on glutamate damage in nerve cells. The polysaccharides were extracted by hot water and purified by sevag method. The model of PC12 cells damaged by glutamate was constructed in vitro. The activity of PC12 neurons was measured by MTT method; the apoptosis of neurons was detected by flow cytometry; the activities of malondialdehyde, catalase, superoxide dismutase and glutathione peroxidase were detected by kit. The optimum extraction method was obtained when the ratio of material to liquid was 1∶30, the extraction time was 3 hours and the temperature was 120 ℃. Agaricus blazei murrill polysaccharide could significantly improve the survival rate of nerve cells injured by glutamate, reduce the production of malondialdehyde, and increase the activities of catalase, superoxide dismutase and glutathione peroxidase. Agaricus blazei murrill polysaccharide has a significant protective effect on glutamate-induced neuronal injury, and its mechanism may be related to antagonizing glutamate-induced oxidative stress.
【Fund】: 宿州学院自然科学重点项目(2017yzd11);宿州学院宿州市农业科学院实践教育基地项目(szxy2018xxhz02);宿州学院科研平台项目(2017ykf05);; 安徽省自然科学重点项目(KJ2017A441)
【CateGory Index】: TS201.2
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 SONG Xiu-yun;HU Jin-feng;SUN Ming-na;LIU Gang;CHEN Nai-hong;Neuroscience Center, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College;;Neuroprotective effect screening and the mechanism of 10 kinds of coumarin derivatives[J];药学学报;2015-06
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3 WANG Li-juan;ZHANG Yan-qing;WANG Yong;CAO Jin-yan;LI Na;Tianjin Key Laboratory of Food Technology;School of Biotechnology and Food Science,Tianjin University of Commerce;;Immune-Enhancing Effects and Acute Toxicity of Agaricus blazei Polysaccharide in Mice[J];食品科学;2014-13
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 MA Tianyu;JIANG Guozhe;HAN Chunji;LIU Liping;YU Duwen;Department of Preventive Medicine,College of Medical Sciences,Yanbian University;Department of Immunology and Pathogenic Biology,College of Medical Sciences,Yanbian University;Department of Physiology,College of Medical Sciences,Yanbian University;Department of Pharmacy,College of Pharmacy,Chia Nan University;Fun Sun Medical and Pharmaceutical Technology Co.,Ltd.;;Influence of Rhodiolasachalinensis and Codonopsispilosula mixture in immune function of mice[J];吉林大学学报(医学版);2015-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LIN Li-li;WANG Dong;Department of Pharmacology,Wuxi Higher Health Vocational Technology School;Department of Medcine,Chinese PLA General Hospital;;Research progress of clinical transformation status on neuroprotective agents[J];中国临床药理学杂志;2013-11
2 XIE Hao-gui,WEI Ming-qian,CHEN Mei-zhen*,WEN Xiao-bo,LIAO Xu-biao,PANG Si-wei(College of Science,Shantou University,Shantou 515063,China);Synergistic Antitumor Activity in vitro of Polysaccharides from Three Different Matrices[J];食品科学;2013-15
3 WANG Jun-qiao,NIE Shao-ping,YU Qiang,YIN Peng-fei,GAO Jiang-yu,ZHANG Yi-liang,XIE Ming-yong(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China);Immune Modulation and Antioxidation Activity of Polysaccharides from Ganoderma atrum in Immunosuppressed Mice[J];食品科学;2012-23
4 WANG Meizhu,CAO Yuekun,HAN Qiuju(College of Environmental and Biological Engineering,Liaoning Shihua University,Fushun 113001,Liaoning,China);Two Extraction Methods of Lentinan Polysaccharide[J];氨基酸和生物资源;2012-02
5 YU Lei,WANG Baoqi,ZHANG Huafeng,YANG Yun(Department of Pharmacy,Henan College of Traditional Chinese Medicine,Zhengzhou 450008,China);Process Research of Decoloration and Deproteinization of Caulis Polygoni Multiflori Polysaccharides[J];中药新药与临床药理;2012-02
6 SONG Li-hua1,3,XIAO Wei2,3,LU Li-li1,3,WANG Zhen-zhong2,3,JIANG Hua2,3,ZHANG Yi2,3,ZHU Ke-jin2,3 1.Nanjing University of Chinese Medicine,Nanjing 210000,China 2.Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China 3.State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process,Lianyungang 222001,China;Extracting technology optimization of polysaccharides from Panax ginseng by orthogonal test[J];中草药;2012-02
7 ZHOU Bao-zhen1,2,XIAO Ya-ping1,2,NIU Jun-feng1,2(1Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry,Xi'an 710062,China;2Life Science College,Shaanxi Normal University,Xi'an 710062,China);Isolation,purification and analysis of polysaccharide of Gynostemma pentaphyllum polysaccharide by IR,GC[J];中华中医药杂志;2012-01
8 JIA Wei1,2,FAN Hua2,(1.National Engineering Research Centre of Edible Fungi,Key Laboratory of Applied Mycological Resources and Utilization,Ministry of Agriculture,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;2.Institut fur Molekularbiologie und Biochemie,Charite Universitat-Medicine Berlin,Berlin D-14195,German);Effect of Active Polysaccharide Fraction from Agaricus blazei Murill on Mouse Macrophages[J];食品科学;2011-11
9 HUANG Xiao-wei(College of Biochemical Engineering,Beijing Union University,Beijing 100023,China);Unconventional Extraction of Polysaccharides from Medicinal Plants[J];北京联合大学学报(自然科学版);2011-01
10 XIA Yong-gang,LIANG Jun,YANG Bing-you,WANG Qiu-hong,KUANG Hai-xue(Key Laboratory of Chinese Materia Medica(Heilongjiang University of Chinese Medicine),Ministry of Education,Heilongjiang key Laboratory of TCM Pharmacodynamic Material Bases,Harbin 150040,China);Determination of Uronic Acid Contents in Polysaccarides from the Stems of Ephedra Sinica Stapf[J];中医药学报;2011-01
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