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《Optical Technique》 2005-03
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Method achieving fluorescence lifetime micro-image

LIN Zi-yang, NIU Han-ben, GUO Bao-ping, QU Jun-le, PENG Wen-da, TIAN Jing-dong(Institute of Optoelectronics, Shenzhen University, Key Laboratory of Opto-electruonics Devices and Systems (Shenzhen University), Ministry of Education, Shenzhen 518060, China)  
Laser pulses of 120fs duration and 76MHz repetition rate were brought in exciting light pathway of laser scanning microscope and the sample of fluorescent tags was excited, using the scanning system of laser scanning microscope. The fluorescence excited by exciting light was brought in external detected port from fluorescence detection pathway. The fast photomultiplier was connexted on the port. The signal of the fast photomultiplier was sent to time_correlated single photon counting and time_correlated fluorescence intensity image was got. Than, the computer processes the image and the fluorescence lifetime image was obtained. The fluorescence lifetime of cyan fluorescent protein(CFP) and yellow fluorescent protein(YFP) with the system were measured. The measurement of fluorescence resonance energy transfer with CFP and YFP was achieved. With the experiments that the fluorescence lifetime micro_image measurement can be well done by combining laser scanning microscope with advanced method of fluorescence lifetime measurement is showed.
【Fund】: 国家自然科学基金重点项目(No.60138010)
【CateGory Index】: O432.2
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