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《Guangxi Agricultural Sciences》 2008-01
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Extraction and purification of total DNA of soil microorganisms in different environments

WU Hong-ping1,ZHENG Fu-cong2(1.College of Environment and Plant Protection,Hainan University,Danzhou,Hainan 571737,China;2.Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China)  
In this experiment,the total DNA of soil microorganisms from forest land,factory area and vegetable plot were extracted by high salt buffer solution of SDS extraction method.The results showed that the extracted DNA length of soil microorganisms was over 23.1 kb by this method.The DNA amount and purity of different soil microorganisms were different,the extracted DNA amount was 53.55-579.93 μg per gram dry soil,and the extracted DNA amount of soil microorganisms in original forest was the highest.The crude extracted DNA of soil microorganisms was purified by phenol-chloroform and column humic acid,then purified DNA could be used as templates for PCR amplification,and corresponding fragments could be amplified by using primers of bacterial 16S rDNA gene.
【Fund】: 中央公益性科研院所基本科研业务专项(2007hzslJ011)
【CateGory Index】: S154
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