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《Journal of Southern Agriculture》 2011-01
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Monitoring of classical swine fever virus E2 protein antibody levels in weaned piglets

WU Qi-biao, TIAN Juan-juan, TAN Chun-ping, HUANG Bai-hua, LIU Wen-juan, LU Qin-zhang * (College of Animal Science and Veterinary Medicine, Guangxi University, Nanning 530005, China)  
[Objective]The present experiment was conducted to develop a direct and reliable detection method for classical swine fever (CSF) antibody level of weaned piglets. [Method]Ninety serum samples were collected from weaned piglets of different ages from a swine piggery in Guangxi. The piglets were immunized before sucking colostrums. All the samples were monitored for E2 antibody and classical swine fever virus (CSFV) antibody using indirect enzyme-linked immuno sorbent assay (ELISA) and indirect hemagglutination (IHA) test. [Result]Out of 90 samples, 86, accounting for 95.56% of the total samples, showed positive results to indirect ELISA test. The antibody titer of 88 serum samples was above 1∶16 (including 1∶16) in IHA test, which accounted for 97.78% of the total samples. [Conclusion]The results indicated that the serum of piglets had protective anti-E2 antibody, which was found to be available for monitoring the CSFV immunization level using detection of serum antibody level of piglets using indirect ELISA.
【Fund】: 广西科技攻关项目(桂科攻0537008-3)
【CateGory Index】: S858.28
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【Citations】
Chinese Journal Full-text Database 10 Hits
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【Co-citations】
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