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《Journal of Guangxi Normal University(Natural Science Edition)》 2016-01
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Simultaneously Determination of Three Effective Components in Swertia tetraptera Maxim.

ZHAO Ying;LIU Xiaocui;LIAO Zhiming;LU Yongchang;College of Pharmacy,Qinghai University for Nationalities;Key Laboratory of Plant Resources of Qinghai-Tibet Plateau in Chemical Research,Qinghai University for Nationalities;Key Laboratory of Pharmaceutical Analysis in Qinghai Province;  
RP-HPLC was used to determine the quantity and quality of swertiamarin,gentiopicroside and sweroside in Swertia tetraptera Maxim.The results showed that three compounds were base-isolated.The linear ranges of swertiamarin,gentiopicroside and sweroside were 0.052~1.030μg(R=0.999 8),0.060~1.200μg(R=0.999 9),and 0.050~0.99μg(R=0.999 8)respectively,the average recoveries were 99.1%(RSD=1.94%),101.5%(RSD=2.05%),and 96.0%(RSD=1.94%)respectively.This method is simple,rapid and reliable with good reproducibility,and can be used as the determination method for swertiamarin,gentiopicroside and sweroside in Swertia tetraptera Maxim.
【Fund】: 国家自然科学基金资助项目(81160554);; 青海省青藏高原植物化学重点实验室发展专项(2014-Z-Y13)
【CateGory Index】: O657.72;R284.1
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