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《Journal of Guangxi Normal University(Natural Science Edition)》 2016-01
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Determination of Six Kinds of Active Ingredients from Gentiana algida Pall by CZE

BAI Xue;ZENG Qingyi;ZHU Kuide;LU Yongchang;College of Pharmacy,Qinghai University for Nationalities;Key Laboratory of Plant Resources of Qinghai-Tibet Plateau in Chemical Research;Key Laboratory of Pharmaceutical Analysis in Qinghai Province;  
To establish a CZE method for simultaneous determination of gentiopicroside,loganic acid,oleanic acid,uosolic acid,isoorientin and mangiferin in the herbs of Gentiana algida Pall.70mmol/L borax-20%aetonitrile was used as buffer solution(pH=11).The separation was performed on an uncoated fused silica capillary(64.5cm×50μm,56 cm of effective length),at voltage of 22 kV.The detection wavelength was 200 nm,and the sample was injected at 50mbar×5s.As a result,the calibration curves of the six active ingredients showed good linearity(r0.999 5)in the ranges of the tested concentrations,and the average recoveries of the method were between 97.21% ~98.12%.The method is simple,accurate and can be used for the quality evaluation and control of Gentiana algida Pall.
【Fund】: 国家自然科学基金资助项目(81160554);; 青海省重点实验室发展专项资金(2015-Z-Y09)
【CateGory Index】: R284.1
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