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《Journal of Guangxi Normal University(Natural Science Edition)》 2016-01
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Effect of Starvating and Refeeding on Relative Expression of FSRP-1,FSRP-3 and PepT1 Gene in Mandarin Fish(Siniperca chuatsi)

WANG Jianhua;LI Yulong;CHEN Dunxue;ZHU Xin;LIU Zhixing;ZHANG Jianshe;CHU Wuying;BIN Shiyu;College of Life Science,Guangxi Normal University;Department of Bioengineering and Environmental Science,Changsha University;  
To investigate postprandial changes in transcript abundance following a single satiating meal in mandarin fish(Siniperca chuatsi)going on a fast for 1week.The expression of FSRP-1and FSRP-3gene in fast myotomal muscle,as well as the PepT1 gene in small intestine were determined using quantitative real-time PCR at the following postprandial time points:0,1,3,6,12,24,48 and 96h.The results indicated that:FSRP-1transcripts were sharply up-regulated within 12 hof refeeding(P0.05),the transcripts were maintained in the high level from 12 hto 48h,then it was significant downregulation in response to feeding;The FSRP-3gene expression was increased significantly(P0.05)in1hpostprandially,and reached peak expression levels at 12 hpostprandially,and decreased significantly after 48hpostprandially(P0.05).The expression level of FSRP-3at 12 h was 5times higher compared with which at 1 h;The PepT1 gene had decreased expression in 6 h after the meal administration significantly(P0.05),and reached maximal expression levels at 48 hpostprandially.FSRP-1,FSRP-3and PepT1 were absolutely necessary,associated with growth,which were greatly influence by starvation and refeeding.
【Fund】: 国家自然科学基金资助项目(31472256 31560718 31230076);; 湖南省教育厅开放基金资助项目(15K011)
【CateGory Index】: S917.4
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