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《Journal of Guangxi Normal University(Natural Science Edition)》 2017-03
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The Preliminary Screening of Hypaphorine High-accumulation Strain by Using the Quick Fluorescent Method

LIU Yi;YE Xuemei;XIAO Miyun;L Lijun;HOU Chengyou;LU Zujun;College of Life Science,Guangxi Normal University;Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection,Ministry of Education;Guilin Runtai Biological Technology Co.LTD;  
Hypaphorine is an indole alkaloid identified as one of active constituents in some Chinese medicinal herbs.Pisolithus tinctorius is a Sclerodermatales macro-fungi which accumulate hypaphorine in its hyphae.Hypaphorine's native fluorescent radiation is strong enough,but tryptophan has almost the same fluorescence wavelength as that of Hypaphorine,which will cause serious interference when the hypaphorine was measured.A quick tryptophan-detective Fluorescent spectrometry method has been completed,and one Pisolithus tinctorius strain containing 3.39 nmol· mg~(-1) hypaphorine has been screened by using this method.
【Fund】: 国家自然科学基金(31060120);; 广西自然科学基金(13GXNSFAA019059);; 广西科技项目(桂科攻14124004-3-8 桂科攻14125006-81);; 广西大学生创新创业训练项目(201610602054)
【CateGory Index】: Q93
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【Citations】
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Yi;YE Xuemei;XIAO Miyun;L Lijun;HOU Chengyou;LU Zujun;College of Life Science,Guangxi Normal University;Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection,Ministry of Education;Guilin Runtai Biological Technology Co.LTD;;The Preliminary Screening of Hypaphorine High-accumulation Strain by Using the Quick Fluorescent Method[J];广西师范大学学报(自然科学版);2017-03
2 CAI Jin-jin;CHEN Lu;School of Clinical Medical,Teaching Hospital,Chengdu University of Traditional Chinese Medicine(TCM);Chengdu University of TCM,Key Laboratory of Standardization of Chinese Herbal Medicine,Ministry of Education,Key Laboratory of Systematic Research,Development and Utilization of Chinese Medicine Resources in Sichuan Province,Key Laboratory Breeding Base of Co-founded by Sichuan Province and Ministry of Science and Technology;;Analysis on Variation of Fatty Oil Components in Vaccariae Semen,Cassiae Semen and Ziziphi Spinosae Semen Before and After Stir-frying by GC-MS[J];中国实验方剂学杂志;2017-15
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