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《Journal of Guangxi Normal University(Natural Science Edition)》 2018-04
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Immobilization of Lipase Using Domestic Epoxy Resin LXEP-120

XU Shan;LI Renqiang;ZHANG Jifu;ZHANG Yun;SUN Aijun;HU Yunfeng;Department of Biotechnology,Jinan University;Guangdong Provincial Hospital of Chinese Medicine;CAS Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences;Guangdong Key Laboratory of Marine Materia Medica,South China Sea Institute of Oceanology,Chinese Academy of Sciences;  
The immobilization of lipase from procine pancrestic was performed by using domestic epoxy resin LXEP-120.The immobilization conditions were optimized,and the enzymatic properties of immobilized lipase and free lipase were also compared.The optimal immobilization conditions were as follows:using 1.0 mol/L pH6.0 potassium phosphate buffer,the quantity of epoxy resin being 5.0 g,the appropriate temperature being 22 ℃,and the immobilized time being 12 h.Under above optimized conditions,the activity of immobilized lipase was about 400 U/g,and the recovery rate of the enzymatic activity of the immobilized lipase reached 92.79%.The immobilized lipase retained 70% of its initial enzymatic activity after repeated usage for 10 times.The optimal reaction pH(pH 8.0)was the same as that of free lipase,and the optimal reaction temperature(45℃)was 5℃ higher than that of free lipase.The thermal-stability and operating-stability reuse rate of immobilized lipase were significantly better than those of free lipase.Additionally,immobilized lipase exhibited very good storage stability.The study shows that domestic epoxy resins LXEP-120 possesses great potential in the immobilization of industrial enzymes.
【Fund】: 中国科学院战略性先导科技专项(XDA11030404);; 广东省海洋渔业科技攻关与研发方向项目(A201701C12);; “科学”号高端用户项目(KEXUE2018G05);; 国家自然科学基金(21302199);; 广州市科技计划项目(201510010012)
【CateGory Index】: Q814.2
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