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《Journal of Guangxi Normal University(Natural Science Edition)》 2018-04
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Isolation and Identification of a Bacterium Producing Natural Blue Pigment

XIAO Miyun;RUAN Chujin;CHEN Shoukun;LIU Yuhua;LU Zujun;College of Life Science,Guangxi Normal University;Key Laboratory of Ecological and Environmental Protection for Rare and Endangered Animals and Plants(Guangxi Normal University);Institute of Microbiology,Chinese Academy of Sciences;  
In order to isolate and identify a bacterium producing natural blue pigment,surface sterilization method was applied to isolate producing natural blue pigment target strain from the root of Bovine Millettia specisoa Champ.16 SrDNA sequence of the target strain was amplified by PCR with universal primer 27 F/1492 R,and the phylogenetic tree was constructed by comparing and analyzing the sequence.The morphology of the bacterium was observed at the same time.The target strain was identified by the GEN III MicroStation microorganism automatic identification system.The results of the sequence alignment showed that the similarity rate of target strain and Pseudomonasaeruginosa was99% and the phylogenetic tree showed that the target strain was on the same branch of the P.aeruginosa.Morphological identification revealed that it was rod-shaped and Gram negative,and Biolog identification showed that it was P.aeruginosa.The target strain was identified as P.aeruginosa by the combined results of molecular,morphology and Biolog identification and named as 2016 NX1.
【Fund】: 国家自然科学基金(31060120);; 广西研究生教育创新计划项目(XYCSZ2017070)
【CateGory Index】: Q939
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