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《ACTA OPTICA SINICA》 2000-04
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Ultra-High Sensitivity Fluorescence Microscope and Its Application in Biology

Li Qin (Department of Modern Applied Physics, Tsinghua University, Beijing 100084) Yu Xin Hu Xinqi Ma Yu (Opto Electronic Department, Beijing Institute of Technology, Beijing 100081) Zhang Tongcun Cao Enhua (Institute of Biophysics, The Chi  
Based on the digital high sensitivity fluorescence microscope, the ultra high sensitivity fluorescence microscope is developed. The system can enhance the fluorescence intensity more than 10 6 and get the photon sensitive image of ultra weak fluorescence. The accurate position of ultra weak fluorescence can be confirmed by image fusion methods. Because the speed of image acquired system is 25 frame/s, the dynamic characters of fluorescence intensity can be investigated. Using the novel microscope to study the apoptosis of MGC 803 cells induced by As 2O 3, the density of Ca 2+ in apoptotic cells obviously increased. Ca 2+ is probably one of important roles in the process of MGC 803 apoptosis induced by As 2O 3.
【Fund】: 国家科委 86 3高科技项目;; 国家自然科学基金资助项目!(6 984 80 0 1)
【CateGory Index】: TH74
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