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《Acta Optica Sinica》 2000-12
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A Study of Time Domain 2-Dimensional Fluorescence Lifetime Microscopic Measurement

Qu Junle Niu Hanben Li Ji Zhao Huijuan Chen Xiaoying (Xi′an Institute of Optics and Precision Mechanics, The Chinese Academy of Sciences, Xi′an 710068) (Received 10 May 1999; revised 12 July 1999)  
A new technique of time domain 2 dimensional fluorescence lifetime microscopic measurement is presented. A fluorescence lifetime imaging microscope system and data processing method of this technique are described. Reference sample is used to test the system. Experimental results show that the temporal resolution of the system is 2 ps. When magnification of the microscope is 100, the spatial resolution is 8 μm. When a finer mask and a micro shift mechanical system are used, spatial resolution of less than 1 μm can be obtained.
【CateGory Index】: TH74
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1 Liu Lixin~ 1,2,3 Qu Junle~3 Lin Ziyang~3 Chen Danni~3 Hu Tao~3 Guo Baoping~3 Niu Hanben~31 Xi'an Institute of Optics and Precision Mechanics, The Chinese Academy of Sciences, Xi'an 7100682 Graduate School of the Chinese Academy of Sciences, Beijing 1000493 Institute of Optoelectronics, Shenzhen University, Shenzhen 518060;A High Repetition Rate Picosecond Streak Camera for Two-Photon Excited Fluorescence Lifetime Microscopic Imaging[J];Acta Optica Sinica;2006-03
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2 Zhang YoujunTang Weizhong (Department of Electrooics Engineering,Zhengzhou University,Zhengzhou 450052 )(Zhejiang University) Guo JunweiZhou Hong (Zhengzhou University)(Zhongzhou College);NonContact OpticFiber Temperature Sensor Based on Fluorscence[J];JOURNAL OF TRANSDUCER TECHNOLOGY;1999-03
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4 LIU Ying (Dept.of Science and Technotogy,TV University of Feshan Gaoming, Feshan 528000) LIU Gui Xiong (Dept.of Mechatronic Engineering,South China University of Technology,Guangzhou 510641);The Research on a Ruby Fluorescence Optical Fiber Temperature Sensor[J];OPTICS AND PRECISION ENGINEERING;1998-01
5 Shen Yonghang (Zhejiang University, Department of Physics, Hangzhou 310027) (Received 23 October 1998; revised 09 December 1998);Sapphire Fiber Thermometer Ranging from the Room Temperature to 1800℃[J];ACTA OPTICA SINICA;2000-01
6 Guan Xiaowei Liu Jingru Huang Meisheng Li Zhefu Zhang Zhenrong Liu Jianshang (Northwest Institute of Nuclear Technology, Xi’an 710024);Study on Instantaneous Temperature Measurement in a Flame by LIPF[J];Acta Optica Sinica;2001-03
7 Sun Weimin 1 Zhang Jianzhong 1 Yu Lei 2 Wang Jingfei 1 Xiang Yanrong 1 Zhang Yang 1 Yuan Libo 1 Grattan K T V 3 Sun Tong 3 1 School of Science, Harbin Engineering University, Harbin 150001 2 School of Information and Communication Engineering, Harbin Engineering University, Harbin 150001 3 School of Engineering, City University, London EC1V 0HB, UK;Fitting Method of Fluorescence Lifetime Based on Fast Fourier Transform Arithmetic[J];Acta Optica Sinica;2004-06
8 HU Chun-hai,ZOU Xiao-hong,WANG Yu-tian (Electric Institute of Yanshan University,Hebei Qinhuangdao 066004,China);Research on optical fiber temperature sensor based on fluorescence lifetime for online temperature monitoring for high voltage equipment[J];Industrial Instrumentation & Automation;2004-05
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【Secondary References】
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1 Niu Lihong Liu Jinyuan Peng Wenda Niu Hanben Yang QinlaoLiao Hua Zhou Junlan Zhan Jinsong(Institute of Optoelectronics,Shenzhen University,Shenzhen,Guangdong,518060,China);Microchannel Plate Gated X-Ray Nanosecond Framing Camera[J];Acta Optica Sinica;2008-07
2 Mao Zhengle Wang Chen Cheng Ya(State Key Laboratory of High Field Laser Physics,Shanghai Institute of Optics and Fine Mechanics,Chinese Academy of Sciences,Shanghai 201800,China);Superresolution Far-Field Fluorescence Bio-Imaging:Breaking the Diffraction Barrier[J];Chinese Journal of Lasers;2008-09
3 SHAO Yong-hong1,LI Heng1,2,WANG Yan1,QU Jun-le1,and Hanben NIU1 1)College of Optoelectronic Engineering Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education,Key Laborotory of Optoelectronics Devices and Systems of Guangdong Province,Shenzhen University Shenzhen 518060 P.R.China 2)College of Opt-electro Engineering Xi'an Technological University Xi'an 710032 P.R.China;A fluorescence lifetime spectrometer based on a synchroscan streak camera[J];Journal of Shenzhen University Science and Engineering;2009-04
4 Liu Li_Xin~ 1)2)3) Qu Jun_Le~ 1) Lin Zi_Yang~ 1) Chen Dan_Ni~ 1) Xu Gai_Xia~ 1) Hu Tao~ 1) Guo Bao_Ping~ 1) Niu Han_Ben~ 1) 1)(Institute of Optoelectronics, Shenzhen University, Shenzhen 518060, China)2)(Xi′an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi′an 710068, China)3)(Graduate School of Chinese Academy of Sciences, Beijing 100049, China);Time-resolved two-photon excitation fluorescence spectroscopy[J];Acta Physica Sinica;2006-12
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