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《Acta Optica Sinica》 2017-08
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An Analytical Model for Multi-Wavelength Transmittance Spectra of Bacteria in Water

Hu Yuxia;Zhao Nanjing;Gan Tingting;Duan Jingbo;Yu Huijuan;Meng Deshuo;Liu Jianguo;Liu Wenqing;Key Laboratory of Environment Optics and Technology,Anhui Institute of Optics and Fine Mechanics,Chinese Academy of Sciences;University of Science and Technology of China;Anhui Key Laboratory of Environment Optical Detection Technology;  
Multi-wavelength transmittance spectra of bacteria in water contain lots of information on their characteristics such as structure,constituent and concentration,and the effective extraction of the information is the foundation of rapid identification and detection of bacteria.The transmittance spectrum of Escherichia coli is recorded with a ultraviolet-visible spectrophotometer.According to Mie scattering theory and absorption and scattering properties,bacterial multi-wavelength transmittance spectrum analytical model in 240-900 nm band is constructed.Based on the model,the spectrum in the range of 250-750 nm is analyzed,the related parameters such as volume,diameter,structure and concentration of Escherichia coli cells are obtained,and these parameters are then compared with the literature data or experimental data.It is shown that the proposed multi-wavelength transmission spectrum model can accurately characterize the spectral features of bacteria in water,and the model can provide important parameters for the rapid analysis and detection of bacteria in water.
【Key Words】: spectroscopy multi-wavelength transmission spectrum Mie scattering bacterial microorganism analytical model
【Fund】: 国家自然科学基金(61378041);; 安徽省自然科学基金(1508085JGD02 1608085QF137);; 安徽省科技重大专项(15CZZ04125)
【CateGory Index】: O433.4;Q93
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